Scale-up strategy for yeast single cell oil production for Rhodotorula mucilagenosa IIPL32 from corn cob derived pentosan.

Autor: Banerjee A; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India., Sharma T; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India., Nautiyal AK; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India., Dasgupta D; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India., Hazra S; Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand 247667, India., Bhaskar T; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India., Ghosh D; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India. Electronic address: dghosh@iip.res.in.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2020 Aug; Vol. 309, pp. 123329. Date of Electronic Publication: 2020 Apr 09.
DOI: 10.1016/j.biortech.2020.123329
Abstrakt: This work was aimed to strategically scale-up the yeast lipid production process using Reynolds number as a standard rheological parameter from 50 mL to 50 L scale. Oleaginous yeast Rhodotorula mucilaginosa IIPL32 was cultivated in xylose rich corncob hydrolysate. The fermentation process for growth and maturation was operated in fed-batch with two different C/N ratios of 40 and 60. The hydrodynamic parameters were used to standardize and represent the effect of rheology on the fermentation process. The growth pattern of the yeast was found similar in both shake flask and fermenter with the maximum growth observed at 48 h. The lipid yield increased from 0.4 g/L and 0.5 g/L to 1.3 g/L and 1.83 g/L for 50 mL to 50 L for C/N ratio 40 and 60 respectively. The increase in productivity during the growth phase and lipid accumulation during the maturation phase showed that the scale-up strategy was successful.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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