Toxicity evaluation of nanocrystalline silver-impregnated coated dressing on the life cycle of worm Caenorhabditis elegans.

Autor: Ayech A; Universidade Federal do Rio Grande- FURG, Instituto de Ciências Biológicas (ICB), Programa de Pós-graduação em Ciências Fisiológicas (PPGCF), Rio Grande, RS, Brazil., Josende ME; Universidade Federal do Rio Grande- FURG, Instituto de Ciências Biológicas (ICB), Programa de Pós-graduação em Ciências Fisiológicas (PPGCF), Rio Grande, RS, Brazil., Ventura-Lima J; Universidade Federal do Rio Grande- FURG, Instituto de Ciências Biológicas (ICB), Programa de Pós-graduação em Ciências Fisiológicas (PPGCF), Rio Grande, RS, Brazil., Ruas C; Universidade Federal do Rio Grande- FURG, Escola de Química e Alimentos (EQA), Programa de Pós-graduação em Química Tecnológica e Ambiental (PPGQTA), Rio Grande, RS, Brazil., Gelesky MA; Universidade Federal do Rio Grande- FURG, Escola de Química e Alimentos (EQA), Programa de Pós-graduação em Química Tecnológica e Ambiental (PPGQTA), Rio Grande, RS, Brazil., Ale A; Instituto Nacional de Limnología (INALI), UNL, CONICET, Santa Fe, Argentina., Cazenave J; Instituto Nacional de Limnología (INALI), UNL, CONICET, Santa Fe, Argentina., Galdopórpora JM; Universidad de Buenos Aires (UBA), CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina., Desimone MF; Universidad de Buenos Aires (UBA), CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina., Duarte M; Universidade Federal do Rio Grande- FURG, Instituto de Ciências Biológicas (ICB), Programa de Pós-graduação em Ciências Fisiológicas (PPGCF), Rio Grande, RS, Brazil., Halicki P; Universidade Federal do Rio Grande- FURG, Faculdade de Medicina (FAMED), Programa de Pós-graduação em Ciências da Saúde (PPGCS), Rio Grande, RS, Brazil., Ramos D; Universidade Federal do Rio Grande- FURG, Faculdade de Medicina (FAMED), Programa de Pós-graduação em Ciências da Saúde (PPGCS), Rio Grande, RS, Brazil., Carvalho LM; Universidade Federal de Santa Maria (UFSM), Departamento de Química, Programa de Pós-graducação em Ciências Farmacéuticas, Santa María, RS, Brazil., Leal GC; Universidade Federal de Santa Maria (UFSM), Departamento de Química, Programa de Pós-graducação em Ciências Farmacéuticas, Santa María, RS, Brazil., Monserrat JM; Universidade Federal do Rio Grande- FURG, Instituto de Ciências Biológicas (ICB), Programa de Pós-graduação em Ciências Fisiológicas (PPGCF), Rio Grande, RS, Brazil. Electronic address: monserrat_jm@furg.br.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2020 Jul 01; Vol. 197, pp. 110570. Date of Electronic Publication: 2020 Apr 17.
DOI: 10.1016/j.ecoenv.2020.110570
Abstrakt: In recent times, however, due to the emergence of bacterial strains with resistance to conventional antibiotics, silver has again gained attention as an alternative for developing new efficient bactericides, including the use of silver nanoparticles (AgNPs). However, the improper disposal of these items after use may cause toxicological effects on organisms in the environment. To evaluate the potential environmental hazard of nanosilver-coated dressings, the nematode Caenorhabditis elegans was chosen as a test organism. The assays were conducted in 24-well plates that contain four different sizes of coated dressing to obtain different concentrations. L1 and L4 C. elegans larval stages were exposed to these nanosilver concentrations. Dressing cutouts were arranged between two layers of agar for 3 days and Escherichia coli (OP 50 strain) was added as food source for the worms. After the exposure period, growth, reproduction, fertility, silver concentration in the medium and the concentration of reactive oxygen species (ROS) in the worms were evaluated. Scanning and transmission electron microscopy analyses were performed on the coated dressings, as well as analyses of zeta potential, ionic release and antibacterial power in two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus). It was verified the antibacterial power of the coated dressing, in both bacteria strains tested. Characterization of the coated dressing indicated heterogeneous nanoparticles, as well as distinct zeta potentials for the medium in water and saline medium (0.9% NaCl). L1 larval worms exposed to nanosilver-coated dressing showed a high ROS concentration and reductions in growth, fertility and reproduction. Worms exposed to the coated dressing during the L4 stage showed almost no response. Overall, the obtained results indicate the potential environmental hazard of nanosilver-coated dressings.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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