Biogenic silver nanoparticles: In vitro and in vivo antitumor activity in bladder cancer.

Autor: Ferreira LAB; Nano-cell Interactions Lab., Department Biochemistry & Tissue Biology, Biology Institute, University of Campinas, Campinas, SP, Brazil., Garcia-Fossa F; Nano-cell Interactions Lab., Department Biochemistry & Tissue Biology, Biology Institute, University of Campinas, Campinas, SP, Brazil., Radaic A; Nano-cell Interactions Lab., Department Biochemistry & Tissue Biology, Biology Institute, University of Campinas, Campinas, SP, Brazil., Durán N; Nanomedicine Research Unit (Nanomed), Center for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André, Brazil; Laboratory of Urogenital Carcinogenesis and Immunotherapy, Department of Structural and Functional Biology, University of Campinas, Campinas, SP, Brazil., Fávaro WJ; Laboratory of Urogenital Carcinogenesis and Immunotherapy, Department of Structural and Functional Biology, University of Campinas, Campinas, SP, Brazil., de Jesus MB; Nano-cell Interactions Lab., Department Biochemistry & Tissue Biology, Biology Institute, University of Campinas, Campinas, SP, Brazil. Electronic address: dejesus@unicamp.br.
Jazyk: angličtina
Zdroj: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2020 Jun; Vol. 151, pp. 162-170. Date of Electronic Publication: 2020 Apr 18.
DOI: 10.1016/j.ejpb.2020.04.012
Abstrakt: Bladder cancer is the fifth most common disease in the United States, and the treatment and alternatives for patients have not changed in the last decades. Silver nanoparticles (AgNP) have been used in the treatment of various cancer, mainly because of the antineoplastic activity; however, their use and the molecular mechanisms towards bladder cancer still unexplored. Therefore, this work aims to evaluate the in vitro and in vivo antitumoral mechanisms of biogenic silver nanoparticles synthesized from Fusarium sp. First, AgNP showed cytotoxicity in a dose- and time-response relationship and detailed analysis demonstrated the induction of cell death via apoptosis, also inhibiting cell migration and proliferation in bladder carcinoma cell line 5637. Next, it was evaluated the antitumoral activity of AgNP against non-muscle invasive bladder cancer (NMIBC). Bladder cancer was chemically induced with N-methyl-N-nitrosourea (MNU) on C57BL/6JUnib female mice and treated by intravesical route with AgNP concentrations of 0.5, 0.2, and 0.05 mg/mL. Finally, treatment with AgNP (0.05 mg/mL) led to 57.13% of tumor regression, with 14.28% of the animals showing normal urothelium, and 42.85% showing flat hyperplasia, considered to be a benign lesion. Overall, these findings demonstrated that AgNP might be a cost-effective alternative and promising candidate for the treatment of bladder cancer.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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