The Comparative Toxicity of Xanthones and Tannins in Mangosteen ( Garcinia mangostana Linn.) Pericarp Extract against BHK-21 Fibroblast Cell Culture.

Autor:	Puteri FH; Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Widjaja J; Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Cahyani F; Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Mooduto L; Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Wahjuningrum DA; Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Jazyk:	angličtina
Zdroj:	Contemporary clinical dentistry [Contemp Clin Dent] 2019 Apr-Jun; Vol. 10 (2), pp. 319-323.
DOI:	10.4103/ccd.ccd_579_18
Abstrakt:	Objective: The objective of this study is to compare the toxicity level of xanthones and tannins derived from mangosteen pericarp extract at specific concentrations against BHK-21 fibroblast cell cultures. Methods: Mangosteen was extracted using a maceration method with ethanol 96%. Xanthones were isolated from the chloroform extract, whereas tannins were isolated using acetone alcohol and serial diluted to 100% concentration. Toxicity levels were monitored after 24 h using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay technique by ELISA reader at 620 nm. Results: Viable cells of BHK-21 against xanthone concentration began to decrease (40.24%) at 3.98% xanthones, whereas viable cells of BHK-21 against tannin concentration began to decrease (68.06%) at 2.2% tannins. Conclusion: It is suggested that tannins were more toxic than the xanthones derived from mangosteen pericarp. Competing Interests: There are no conflicts of interest. (Copyright: © 2020 Contemporary Clinical Dentistry.)
Databáze:	MEDLINE
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