| Autor: |
Matheson A; Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada., Regmi SC; Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada., Martin-Alarcon L; Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada., Jay GD; Department of Emergency Medicine - Warren Alpert Medical School & School of Engineering, Brown University, Providence, RI, USA., Scott WM; Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.; Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada., Schmidt TA; Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.; Biomedical Engineering Department, University of Connecticut Health Center, Farmington, CT, USA. |
| Abstrakt: |
Purpose : In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently. The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis. Materials and Methods : Equine samples of SF (from various joints) and blood were collected at the point-of-care. Proteoglycan-4 concentrations were measured by amplified-luminescence-proximity-assay and enzyme-linked-immunosorbent-assay in SF and serum, respectively. Molecular-weight of hyaluronan was characterized by agarose-gel-electrophoresis, and concentrations were measured by enzyme-linked-immunosorbent-assay kit. Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test. Results : SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis. SF and serum proteoglycan-4 concentrations were correlated in normal horses (r 2 = 0.85, p < 0.05), but not in clinical groups. Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal. Conclusion : Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum. Serum proteoglycan-4 and hyaluronan concentrations alone may not be useful biomarkers for equine joint-injury or disease. |
