Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation.

Autor:	Knudsen JR; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark; Laboratory of Microsystems 2, Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne, Batiment BM, 1015, Lausanne, Switzerland., Steenberg DE; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Hingst JR; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Hodgson LR; School of Biochemistry, University of Bristol, Biomedical Sciences Building, University Walk, BS8 1TD, Bristol, United Kingdom., Henriquez-Olguin C; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Li Z; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Kiens B; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Richter EA; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Wojtaszewski JFP; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark., Verkade P; School of Biochemistry, University of Bristol, Biomedical Sciences Building, University Walk, BS8 1TD, Bristol, United Kingdom., Jensen TE; Molecular Physiology Section, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Oe, Denmark. Electronic address: tejensen@nexs.ku.dk.
Jazyk:	angličtina
Zdroj:	Molecular metabolism [Mol Metab] 2020 Sep; Vol. 39, pp. 100998. Date of Electronic Publication: 2020 Apr 17.
DOI:	10.1016/j.molmet.2020.100998
Abstrakt:	Objective: Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown. Methods: Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 h followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4 h post-exercise before and after a 2-hour hyperinsulinemic-euglycemic clamp. Results: A detailed microscopy-based analysis of GLUT4 distribution within seven different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments. Conclusions: An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle. (Copyright © 2020 The Author(s). Published by Elsevier GmbH.. All rights reserved.)
Databáze:	MEDLINE
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