Evaluation of lipid nanoparticles for topical delivery of protocatechuic acid and ethyl protocatechuate as a new photoprotection strategy.

Autor: Daré RG; Department of Pharmacy, State University of Maringá (UEM), Maringá, Paraná 87020-900, Brazil., Costa A; i3S - Institute for Research and Innovation in Health, University of Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; INEB - Institute of Biomedical Engineering, University of Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal., Nakamura CV; Department of Pharmacy, State University of Maringá (UEM), Maringá, Paraná 87020-900, Brazil., Truiti MCT; Department of Pharmacy, State University of Maringá (UEM), Maringá, Paraná 87020-900, Brazil., Ximenes VF; Department of Chemistry, Faculty of Sciences, University of São Paulo (UNESP), Bauru, São Paulo 17033360, Brazil., Lautenschlager SOS; Department of Pharmacy, State University of Maringá (UEM), Maringá, Paraná 87020-900, Brazil. Electronic address: lautenschlager@uem.br., Sarmento B; i3S - Institute for Research and Innovation in Health, University of Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; INEB - Institute of Biomedical Engineering, University of Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; CESPU, IINFACTS - Institute for Research and Advanced Training in Health Sciences and Technologies, Rua Central de Gandra 1317, 4585-116 Gandra, Portugal. Electronic address: bruno.sarmento@ineb.up.pt.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2020 May 30; Vol. 582, pp. 119336. Date of Electronic Publication: 2020 Apr 15.
DOI: 10.1016/j.ijpharm.2020.119336
Abstrakt: Excessive exposure to solar radiation induces injurious effects on human skin. Our previous study evidenced that protocatechuic acid (P0) and ethyl protocatechuate (P2) act against photodamage and photoaging. The present study aimed to develop solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for topical delivery of P0 or P2, as a strategy for photoprotection. Lipid nanoparticles exhibited mean particle size, polydispersity index, zeta potential and association efficiency between 200 and 400 nm, 0.160 to 0.460, -2.2 to -5.2 mV, and 60% to 80%, respectively. The formulations were stable for 3 months when stored at 4 ○ C and 25 ○ C/60% RH. SLNs/NLCs-P0 showed minor cytotoxicity effects compared with SLNs/NLCs-P2, in HaCat (keratinocytes) and HFF-1 (fibroblasts) cell lines. Additionally, bare NLCs exhibited less cytotoxicity effect, compared with bare SLNs. NLCs exhibited a controlled in vitro release of P0 and P2, and were able to protect the compounds against UVB degradation. Ex vivo permeability study showed that NLCs modulated P0 and P2 retention profiles on human skin layers. Furthermore, histological analysis of skin showed that NLCs-P0 did not cause morphological alterations, while NLCs-P2 showed a potential irritation effect in the skin structure. Based on these results, NLCs were considered a potential dermatological nanocarrier for P0 delivery.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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