Differential gene expression in peripheral blood mononuclear cells from children immunized with inactivated influenza vaccine.

Autor:	Alcorn JF; Department of Pediatrics, University of Pittsburgh , Pittsburgh, PA, USA., Avula R; Department of Biomedical Informatics, University of Pittsburgh , Pittsburgh, PA, USA., Chakka AB; Department of Biomedical Informatics, University of Pittsburgh , Pittsburgh, PA, USA., Schwarzmann WE; Department of Biomedical Informatics, University of Pittsburgh , Pittsburgh, PA, USA., Nowalk MP; Department of Family Medicine, University of Pittsburgh , Pittsburgh, PA, USA., Lin CJ; Department of Family Medicine, University of Pittsburgh , Pittsburgh, PA, USA., Ortiz MA; Department of Pediatrics, University of Pittsburgh , Pittsburgh, PA, USA.; Center for Vaccine Research, University of Pittsburgh , Pittsburgh, PA, USA., Horne WT; Department of Pediatrics, University of Pittsburgh , Pittsburgh, PA, USA., Chandran UR; Department of Biomedical Informatics, University of Pittsburgh , Pittsburgh, PA, USA., Nagg JP; Department of Pediatrics, University of Pittsburgh , Pittsburgh, PA, USA., Zimmerman RK; Department of Family Medicine, University of Pittsburgh , Pittsburgh, PA, USA., Cole KS; Center for Vaccine Research, University of Pittsburgh , Pittsburgh, PA, USA., Moehling KK; Department of Family Medicine, University of Pittsburgh , Pittsburgh, PA, USA., Martin JM; Department of Pediatrics, University of Pittsburgh , Pittsburgh, PA, USA.
Jazyk:	angličtina
Zdroj:	Human vaccines & immunotherapeutics [Hum Vaccin Immunother] 2020 Aug 02; Vol. 16 (8), pp. 1782-1790. Date of Electronic Publication: 2020 Apr 16.
DOI:	10.1080/21645515.2020.1711677
Abstrakt:	The human immune response to inactivated influenza vaccine is dynamic and impacted by age and preexisting immunity. Our goal was to identify postvaccination transcriptomic changes in peripheral blood mononuclear cells from children. Blood samples were obtained before and at 3 or 7 days postvaccination with 2016-2017 quadrivalent inactivated influenza vaccine and RNA sequencing was performed. There were 1,466 differentially expressed genes (DEGs) for the Day 0-Day 3 group and 513 DEGs for the Day 0-Day 7 group. Thirty-three genes were common between the two groups. The majority of the transcriptomic changes at Day 3 represented innate inflammation and apoptosis pathways. Day 7 DEGs were characterized by activation of cellular processes, including the regulation of cytoskeleton, junctions, and metabolism, and increased expression of immunoglobulin genes. DEGs at Day 3 were compared between older and younger children revealing increased inflammatory gene expression in the older group. Vaccine history in the year prior to the study was characterized by robust DEGs at Day 3 with decreased phagosome and dendritic cell maturation in those who had been vaccinated in the previous year. PBMC responses to inactivated influenza vaccination in children differed significantly by the timing of sampling, patient age, and vaccine history. These data provide insight into the expected molecular pathways to be temporally altered by influenza vaccination in children.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu