The Use of Genipin as an Effective, Biocompatible, Anti-Inflammatory Cross-Linking Method for Nerve Guidance Conduits.

Autor: Kočí Z; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin 2, D02YN77, Ireland.; Trinity Centre for Biomedical Engineering (TCBE), Trinity College Dublin, Dublin, D02PN40, Ireland.; Advanced Materials and Bioengineering Research (AMBER) Centre, Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, D02YN77, Ireland., Sridharan R; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin 2, D02YN77, Ireland.; Trinity Centre for Biomedical Engineering (TCBE), Trinity College Dublin, Dublin, D02PN40, Ireland.; Advanced Materials and Bioengineering Research (AMBER) Centre, Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, D02YN77, Ireland., Hibbitts AJ; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin 2, D02YN77, Ireland.; Trinity Centre for Biomedical Engineering (TCBE), Trinity College Dublin, Dublin, D02PN40, Ireland.; Advanced Materials and Bioengineering Research (AMBER) Centre, Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, D02YN77, Ireland., Kneafsey SL; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin 2, D02YN77, Ireland.; Trinity Centre for Biomedical Engineering (TCBE), Trinity College Dublin, Dublin, D02PN40, Ireland.; Advanced Materials and Bioengineering Research (AMBER) Centre, Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, D02YN77, Ireland., Kearney CJ; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin 2, D02YN77, Ireland.; Trinity Centre for Biomedical Engineering (TCBE), Trinity College Dublin, Dublin, D02PN40, Ireland.; Advanced Materials and Bioengineering Research (AMBER) Centre, Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, D02YN77, Ireland., O'Brien FJ; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin 2, D02YN77, Ireland.; Trinity Centre for Biomedical Engineering (TCBE), Trinity College Dublin, Dublin, D02PN40, Ireland.; Advanced Materials and Bioengineering Research (AMBER) Centre, Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, D02YN77, Ireland.
Jazyk: angličtina
Zdroj: Advanced biosystems [Adv Biosyst] 2020 Mar; Vol. 4 (3), pp. e1900212. Date of Electronic Publication: 2020 Feb 06.
DOI: 10.1002/adbi.201900212
Abstrakt: A number of natural polymer biomaterial-based nerve guidance conduits (NGCs) are developed to facilitate repair of peripheral nerve injuries. Cross-linking ensures mechanical integrity and desired degradation properties of the NGCs; however, common methods such as formaldehyde are associated with cellular toxicity. Hence, there is an unmet clinical need for alternative nontoxic cross-linking agents. In this study, collagen-based NGCs with a collagen/chondroitin sulfate luminal filler are used to study the effect of cross-linking on mechanical and structural properties, degradation, biocompatibility, and immunological response. A simplified manufacturing method of genipin cross-linking is developed, by incorporating genipin into solution prior to freeze-drying the NGCs. This leads to successful cross-linking as demonstrated by higher cross-linking degree and similar tensile strength of genipin cross-linked conduits compared to formaldehyde cross-linked conduits. Genipin cross-linking also preserves NGC macro and microstructure as observed through scanning electron microscopy and spectral analysis. Most importantly, in vitro cell studies show that genipin, unlike the formaldehyde cross-linked conduits, supports the viability of Schwann cells. Moreover, genipin cross-linked conduits direct macrophages away from a pro-inflammatory and toward a pro-repair state. Overall, genipin is demonstrated to be an effective, safe, biocompatible, and anti-inflammatory alternative to formaldehyde for cross-linking clinical grade NGCs.
(© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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