Enhancing CO 2 -Valorization Using Clostridium autoethanogenum for Sustainable Fuel and Chemicals Production.

Autor: Heffernan JK; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Saint Lucia, QLD, Australia., Valgepea K; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Saint Lucia, QLD, Australia.; ERA Chair in Gas Fermentation Technologies, Institute of Technology, University of Tartu, Tartu, Estonia., de Souza Pinto Lemgruber R; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Saint Lucia, QLD, Australia., Casini I; Center for Applied Geosciences, University of Tübingen, Tübingen, Germany., Plan M; Queensland Node of Metabolomics Australia, The University of Queensland, Saint Lucia, QLD, Australia., Tappel R; LanzaTech Inc., Skokie, IL, United States., Simpson SD; LanzaTech Inc., Skokie, IL, United States., Köpke M; LanzaTech Inc., Skokie, IL, United States., Nielsen LK; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Saint Lucia, QLD, Australia.; Queensland Node of Metabolomics Australia, The University of Queensland, Saint Lucia, QLD, Australia., Marcellin E; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Saint Lucia, QLD, Australia.; Queensland Node of Metabolomics Australia, The University of Queensland, Saint Lucia, QLD, Australia.
Jazyk: angličtina
Zdroj: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2020 Mar 27; Vol. 8, pp. 204. Date of Electronic Publication: 2020 Mar 27 (Print Publication: 2020).
DOI: 10.3389/fbioe.2020.00204
Abstrakt: Acetogenic bacteria can convert waste gases into fuels and chemicals. Design of bioprocesses for waste carbon valorization requires quantification of steady-state carbon flows. Here, steady-state quantification of autotrophic chemostats containing Clostridium autoethanogenum grown on CO 2 and H 2 revealed that captured carbon (460 ± 80 mmol/gDCW/day) had a significant distribution to ethanol (54 ± 3 C-mol% with a 2.4 ± 0.3 g/L titer). We were impressed with this initial result, but also observed limitations to biomass concentration and growth rate. Metabolic modeling predicted culture performance and indicated significant metabolic adjustments when compared to fermentation with CO as the carbon source. Moreover, modeling highlighted flux to pyruvate, and subsequently reduced ferredoxin, as a target for improving CO 2 and H 2 fermentation. Supplementation with a small amount of CO enabled co-utilization with CO 2 , and enhanced CO 2 fermentation performance significantly, while maintaining an industrially relevant product profile. Additionally, the highest specific flux through the Wood-Ljungdahl pathway was observed during co-utilization of CO 2 and CO. Furthermore, the addition of CO led to superior CO 2 -valorizing characteristics (9.7 ± 0.4 g/L ethanol with a 66 ± 2 C-mol% distribution, and 540 ± 20 mmol CO 2 /gDCW/day). Similar industrial processes are commercial or currently being scaled up, indicating CO-supplemented CO 2 and H 2 fermentation has high potential for sustainable fuel and chemical production. This work also provides a reference dataset to advance our understanding of CO 2 gas fermentation, which can contribute to mitigating climate change.
(Copyright © 2020 Heffernan, Valgepea, de Souza Pinto Lemgruber, Casini, Plan, Tappel, Simpson, Köpke, Nielsen and Marcellin.)
Databáze: MEDLINE
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