Effect of genetic factors on the response to vitamin D 3 supplementation in the VIDARIS randomized controlled trial.

Autor: Slow S; Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand. Electronic address: sandy.slow@otago.ac.nz., Pearson JP; Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand., Florkowski CM; Clinical Biochemistry Unit, Canterbury Health Laboratories, Christchurch, New Zealand., Elder PA; Steroid Unit, Canterbury Health Laboratories, Christchurch, New Zealand., Lewis JG; Steroid Unit, Canterbury Health Laboratories, Christchurch, New Zealand., Kennedy MA; Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand., Murdoch DR; Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Jazyk: angličtina
Zdroj: Nutrition (Burbank, Los Angeles County, Calif.) [Nutrition] 2020 Jul - Aug; Vol. 75-76, pp. 110761. Date of Electronic Publication: 2020 Feb 12.
DOI: 10.1016/j.nut.2020.110761
Abstrakt: Objectives: Supplementation provides the best means of improving vitamin D status; however, individual responses vary partly owing to genetics. The aim of this study was to determine whether 28 single nucleotide polymorphisms (SNPs) in six key vitamin D pathway genes (GC, DHCR7, CYP2 R1, CYP24 A1, CYP27 B1, VDR) were associated with differences in response to supplementation.
Methods: Participants (N = 313; n = 160 vitamin D, n = 153 placebo) were part of VIDARIS (Vitamin D and Acute Respiratory Infections Study), a double-blind, randomized controlled trial involving oral monthly supplementation of either vitamin D 3 (200 000 IU each for the first 2 mo, thereafter 100 000 IU monthly) or placebo for 18 mo. Circulating 25-hydroxyvitamin D (25[OH]D) concentrations at baseline and 2, 6, 12, and 18 mo, and vitamin D binding protein (Gc-globulin) and calculated free 25(OH)D concentrations at baseline and 2 mo were obtained. Multiple regression was used to model associations between genetic variants and 25(OH)D, Gc-globulin, and free 25(OH)D concentrations.
Results: SNPs within GC, CYP2 R1, and CYP27 B1 were associated with 25(OH)D concentrations following supplementation. However, only two GC gene SNPs (rs2282679, rs1155563) were significant after adjustment for multiple testing. This effect disappeared after more than 2 mo of supplementation. None of the SNPs were significantly associated with Gc-globulin concentrations; however, there was a significant interaction with one SNP in DHCR7 (rs12785878), which was associated with reduced free 25(OH)D concentrations in the supplemented arm.
Conclusion: Only variants of GC were associated with 25(OH)D concentrations after supplementation. This effect was modest and disappeared after >2 mo of supplementation, suggesting it may be time/dose-dependent and saturable.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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