Additive manufacturing of biodegradable porous orthopaedic screw.

Autor:	Dhandapani R; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India., Krishnan PD; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India., Zennifer A; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India., Kannan V; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India., Manigandan A; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India., Arul MR; Department of Orthopaedics, UConn Health, Farmington, CT, 06030, USA., Jaiswal D; Department of Biomedical Engineering, Western New England University, Springfield, MA, 01119, USA.; Department of Orthopaedics, UConn Health, Farmington, CT, 06030, USA., Subramanian A; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India., Kumbar SG; Department of Orthopaedics, UConn Health, Farmington, CT, 06030, USA., Sethuraman S; Centre for Nanotechnology & Advanced Biomaterials, SASTRA Deemed University, Thanjavur, 613401, India.
Jazyk:	angličtina
Zdroj:	Bioactive materials [Bioact Mater] 2020 Apr 06; Vol. 5 (3), pp. 458-467. Date of Electronic Publication: 2020 Apr 06 (Print Publication: 2020).
DOI:	10.1016/j.bioactmat.2020.03.009
Abstrakt:	Advent of additive manufacturing in biomedical field has nurtured fabrication of complex, customizable and reproducible orthopaedic implants. Layer-by-layer deposition of biodegradable polymer employed in development of porous orthopaedic screws promises gradual dissolution and complete metabolic resorption thereby overcoming the limitations of conventional metallic screws. In the present study, screws with different pore sizes (916 × 918 μm to 254 × 146 μm) were 3D printed at 200 μm layer height by varying printing parameters such as print speed, fill density and travel speed to augment the bone ingrowth. Micro-CT analysis and scanning electron micrographs of screws with 45% fill density confirmed porous interconnections (40.1%) and optimal pore size (259 × 207 × 200 μm) without compromising the mechanical strength (24.58 ± 1.36 MPa). Due to the open pore structure, the 3D printed screws showed increased weight gain due to the deposition of calcium when incubated in simulated body fluid. Osteoblast-like cells attached on screw and infiltrated into the pores over 14 days of in vitro culture. Further, the screws also supported greater human mesenchymal stem cell adhesion, proliferation and mineralized matrix synthesis over a period of 21 days in vitro culture as compared to non-porous screws. These porous screws showed significantly increased vascularization in a rat subcutaneous implantation as compared to control screws. Porous screws produced by additive manufacturing may promote better osteointegration due to enhanced mineralization and vascularization. Competing Interests: Authors have no conflict of interest. (© 2020 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.)
Databáze:	MEDLINE
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