Mitochondria-Derived H 2 O 2 Promotes Symmetry Breaking of the C. elegans Zygote.

Autor: De Henau S; Center for Molecular Medicine, Molecular Cancer Research, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands., Pagès-Gallego M; Center for Molecular Medicine, Molecular Cancer Research, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands., Pannekoek WJ; Center for Molecular Medicine, Molecular Cancer Research, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands., Dansen TB; Center for Molecular Medicine, Molecular Cancer Research, University Medical Center Utrecht, Heidelberglaan 100, 3584 CG Utrecht, the Netherlands. Electronic address: t.b.dansen@umcutrecht.nl.
Jazyk: angličtina
Zdroj: Developmental cell [Dev Cell] 2020 May 04; Vol. 53 (3), pp. 263-271.e6. Date of Electronic Publication: 2020 Apr 09.
DOI: 10.1016/j.devcel.2020.03.008
Abstrakt: Symmetry breaking is an essential step in cell differentiation and early embryonic development. However, the molecular cues that trigger symmetry breaking remain largely unknown. Here, we show that mitochondrial H 2 O 2 acts as a symmetry-breaking cue in the C. elegans zygote. We find that symmetry breaking is marked by a local H 2 O 2 increase and coincides with a relocation of mitochondria to the cell cortex. Lowering endogenous H 2 O 2 levels delays the onset of symmetry breaking, while artificially targeting mitochondria to the cellular cortex using a light-induced heterodimerization technique is sufficient to initiate symmetry breaking in a H 2 O 2 -dependent manner. In wild-type development, both sperm and maternal mitochondria contribute to symmetry breaking. Our findings reveal that mitochondrial H 2 O 2 -signaling promotes the onset of polarization, a fundamental process in development and cell differentiation, and this is achieved by both mitochondrial redistribution and differential H 2 O 2 -production.
Competing Interests: Declaration of Interests The authors declare no competing interests.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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