Dysregulated KDR and FLT1 Gene Expression in Colorectal Cancer Patients.

Autor: Mohammad Rezaei F; Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.; Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran., Hashemzadeh S; Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran.; General and Vascular Surgery Department of Tabriz University of Medical Sciences, Tabriz, Iran., Ravanbakhsh Gavgani R; Department of biological science, school of natural science, University of Tabriz, Tabriz, Iran., Hosseinpour Feizi M; Department of Animal Biology, University of Tabriz, Tabriz, Iran., Pouladi N; Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran., Samadi Kafil H; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Rostamizadeh L; Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran., Kholghi Oskooei V; Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Taheri M; Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Sakhinia E; Tabriz Genetic Analysis Center (TGAC), Tabriz University of Medical Sciences, Tabriz, Iran.
Jazyk: angličtina
Zdroj: Reports of biochemistry & molecular biology [Rep Biochem Mol Biol] 2019 Oct; Vol. 8 (3), pp. 244-252.
Abstrakt: Background: Colorectal cancer (CRC) is one of the most commonly-diagnosed malignancies throughout the world and the fourth-leading cause of cancer deaths globally. Angiogenesis and the resultant tumor neovascularization is a well-known cancer hallmark. Here we investigated the expression of FLT1 and KDR , the influential genes in angiogenesis regulation, in CRC patients.
Methods: We assessed FLT1 and KDR mRNA expression in 47 CRC samples and matched adjacent noncancerous tissues (ANCT) by quantitative real-time PCR. The Spearmen correlation coefficient and receiver operating characteristic (ROC) curves were also examined.
Results: Both genes were expressed at significantly greater levels in CRC tissues than in ANCT (p < 0.05). A significant association was found between KDR expression and disease stage and lymph status in CRC patients. Furthermore, the Spearman correlation demonstrated a moderate correlation between FLT1 and KDR expression in CRC samples. Finally, ROC curve analysis demonstrated that FLT1 had the greatest sensitivity (85.1%), while the greatest specificity was achieved by a combination of the two genes.
Conclusion: The dysregulated FLT1 and KDR expression, in addition to the observed correlation and ROC curve results, indicate the critical importance of angiogenesis among the cancer pathways in CRC. These data can broaden our current knowledge of angiogenesis in CRC to improve disease diagnosis and patient treatment.
Databáze: MEDLINE
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