Characterization of the Antinociceptive Activity from Stevia serrata Cav.

Autor:	Cordeiro MS; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil., Simas DLR; Institute of Natural Products Research, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil., Pérez-Sabino JF; School of Chemistry, Faculty of Chemical Sciences and Pharmacy, University of San Carlos of Guatemala, Guatemala 01012, Guatemala., Mérida-Reyes MS; School of Chemistry, Faculty of Chemical Sciences and Pharmacy, University of San Carlos of Guatemala, Guatemala 01012, Guatemala., Muñoz-Wug MA; School of Chemistry, Faculty of Chemical Sciences and Pharmacy, University of San Carlos of Guatemala, Guatemala 01012, Guatemala., Oliva-Hernández BE; School of Chemistry, Faculty of Chemical Sciences and Pharmacy, University of San Carlos of Guatemala, Guatemala 01012, Guatemala., da Silva AJR; Institute of Natural Products Research, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil., Fernandes PD; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil., Giorno TBS; Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2020 Apr 07; Vol. 8 (4). Date of Electronic Publication: 2020 Apr 07.
DOI:	10.3390/biomedicines8040079
Abstrakt:	Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (capsaicin- and glutamate-induced licking response) or thermal (hot plate) models of nociception at 10, 30 or 100 mg/kg doses. The mechanism of action was evaluated using two receptor antagonists (naloxone, atropine) and an enzyme inhibitor (L-NAME). The anti-hyperalgesic effect was evaluated using carrageenan-induced nociception and evaluated in the hot plate. Results: All three doses of EO reduced licking response induced by glutamate, and higher doses reduced capsaicin-induced licking. EO also increased area under the curve, similar to the morphine-treated group. The antinociceptive effect induced by EO was reversed by pretreatment of mice with naloxone (1 mg/kg, ip), atropine (1 mg/kg, ip) or L-NAME (3 mg/kg, ip). EO also demonstrated an anti-hyperalgesic effect. The 100 mg/kg dose increased the latency time, even at 1 h after oral administration and this effect has been maintained until the 96th hour, post-administration. Conclusions: Our data suggest that essential oil of S. serrata presents an antinociceptive effect mediated, at least in part, through activation of opioid, cholinergic and nitrergic pathways.
Databáze:	MEDLINE
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