| Autor: |
Chiu JCY; Department of Mechanical Engineering, University of British Columbia, 2054-6250 Applied Science Lane, Vancouver, BC, Canada. hongma@mech.ubc.ca., Teodoro JA, Lee JH, Matthews K, Duffy SP, Ma H |
| Jazyk: |
angličtina |
| Zdroj: |
Lab on a chip [Lab Chip] 2020 May 07; Vol. 20 (9), pp. 1544-1553. Date of Electronic Publication: 2020 Apr 09. |
| DOI: |
10.1039/c9lc01230c |
| Abstrakt: |
The ability to selectively propagate specific cells is fundamentally important to the development of clonal cell populations. Current methods rely on techniques such as limiting dilution, colony picking, and flow cytometry to transfer single cells into single wells, resulting in workflows that are low-throughput, slowed by propagation kinetics, and susceptible to contamination. Here, we developed a method, called selective laser gelation (SLG), to micropattern hydrogels in cell culture media in order to encapsulate specific cells to selectively arrest their growth. This process relies on the inverse gelation of methylcellulose, which forms a hydrogel when heated rather than cooled. Local heating using an infrared laser enables hydrogel micropatterning, while phase transition hysteresis retains the hydrogel after laser excitation. As a demonstration, we used this approach to selectively propagate transgenic CHO cells with increased antibody productivity. More generally, hydrogel micropatterning provides a simple and non-contact method for selective propagation of cells based on features identified by imaging. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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