Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers.

Autor: Bokore FE; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada., Knox RE; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada., Cuthbert RD; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada., Pozniak CJ; Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada., McCallum BD; Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, Canada., N'Diaye A; Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada., DePauw RM; Advancing Wheat Technologies, Swift Current, Canada., Campbell HL; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada., Munro C; Plant and Food Research, Canterbury Agriculture and Science Centre, Lincoln, New Zealand., Singh A; Department of Agronomy, Iowa State University, Ames, IA, United States of America., Hiebert CW; Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, Canada., McCartney CA; Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, Canada., Sharpe AG; Global Institute for Food Security, University of Saskatchewan, Saskatoon, Canada., Singh AK; Department of Agronomy, Iowa State University, Ames, IA, United States of America., Spaner D; Department of Agricultural, Food and Nutritional Science, 4-10N Agriculture-Forestry Centre, University of Alberta, Edmonton, Canada., Fowler DB; Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada., Ruan Y; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada., Berraies S; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada., Meyer B; Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Apr 08; Vol. 15 (4), pp. e0230855. Date of Electronic Publication: 2020 Apr 08 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0230855
Abstrakt: Growing resistant wheat (Triticum aestivum L) varieties is an important strategy for the control of leaf rust, caused by Puccinia triticina Eriks. This study sought to identify the chromosomal location and effects of leaf rust resistance loci in five Canadian spring wheat cultivars. The parents and doubled haploid lines of crosses Carberry/AC Cadillac, Carberry/Vesper, Vesper/Lillian, Vesper/Stettler and Stettler/Red Fife were assessed for leaf rust severity and infection response in field nurseries in Canada near Swift Current, SK from 2013 to 2015, Morden, MB from 2015 to 2017 and Brandon, MB in 2016, and in New Zealand near Lincoln in 2014. The populations were genotyped with the 90K Infinium iSelect assay and quantitative trait loci (QTL) analysis was performed. A high density consensus map generated based on 14 doubled haploid populations and integrating SNP and SSR markers was used to compare QTL identified in different populations. AC Cadillac contributed QTL on chromosomes 2A, 3B and 7B (2 loci), Carberry on 1A, 2B (2 loci), 2D, 4B (2 loci), 5A, 6A, 7A and 7D, Lillian on 4A and 7D, Stettler on 2D and 6B, Vesper on 1B, 1D, 2A, 6B and 7B (2 loci), and Red Fife on 7A and 7B. Lillian contributed to a novel locus QLr.spa-4A, and similarly Carberry at QLr.spa-5A. The discovery of novel leaf rust resistance QTL QLr.spa-4A and QLr.spa-5A, and several others in contemporary Canada Western Red Spring wheat varieties is a tremendous addition to our present knowledge of resistance gene deployment in breeding. Carberry demonstrated substantial stacking of genes which could be supplemented with the genes identified in other cultivars with the expectation of increasing efficacy of resistance to leaf rust and longevity with little risk of linkage drag.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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