Effects of anastrozole on Ki-67 antigen expression in the vaginal epithelium of female rats in persistent estrus.

Autor:	Chagas DC; Programa de Pos Graduacao em Ciencias da Saude, Universidade Federal do Piaui, Teresina, PI, BR., Barros-Oliveira MDC; Programa de Pos Graduacao, Rede Nordeste de Biotecnologia (RENORBIO), Universidade Federal do Piaui, Teresina, PI, BR., Lopes-Costa PV; Programa de Pos Graduacao em Ciencias da Saude, Universidade Federal do Piaui, Teresina, PI, BR., Pereira RO; Programa de Pos Graduacao em Ciencias da Saude, Universidade Federal do Piaui, Teresina, PI, BR., Melo MA; Programa de Pos Graduacao em Ciencias da Saude, Universidade Federal do Piaui, Teresina, PI, BR., Costa-Silva DR; Programa de Pos Graduacao, Rede Nordeste de Biotecnologia (RENORBIO), Universidade Federal do Piaui, Teresina, PI, BR., Borges CS; Hospital Getulio Vargas, Universidade Federal do Piaui, Teresina, PI, BR., Viana JL; Hospital Getulio Vargas, Universidade Federal do Piaui, Teresina, PI, BR., Santos ARD; Programa de Pos Graduacao, Rede Nordeste de Biotecnologia (RENORBIO), Universidade Federal do Piaui, Teresina, PI, BR., Facina G; Departamento Ginecologia, Universidade Federal de Sao Paulo, Sao Paulo, SP, BR., Silva BBD; Programa de Pos Graduacao em Ciencias da Saude, Universidade Federal do Piaui, Teresina, PI, BR.; Hospital Getulio Vargas, Universidade Federal do Piaui, Teresina, PI, BR.
Jazyk:	angličtina
Zdroj:	Clinics (Sao Paulo, Brazil) [Clinics (Sao Paulo)] 2020 Apr 03; Vol. 75, pp. e1643. Date of Electronic Publication: 2020 Apr 03 (Print Publication: 2020).
DOI:	10.6061/clinics/2020/e1643
Abstrakt:	Objectives: Aromatase inhibitors are the first-choice drugs for the treatment of hormone sensitive breast cancer. However, in addition to the scarcity of studies, there are controversies about their effects on vaginal epithelial cell proliferation in rats, especially those in persistent estrus. Methods: To investigate vaginal epithelial cell proliferation by Ki-67 antigen expression, persistent estrus was induced in 42 randomly selected rats. These rats were randomly divided into 2 groups: group I (control, n=21), which received 0.1 mL of propylene glycol (vehicle) daily, and group II (experimental, n=21), which received 0.5 mg/kg or 0.125 mg/day of anastrozole diluted with 0.1 mL of propylene glycol. Results: Light microscopy showed a higher concentration of cells with brown Ki-67 stained nuclei in the control compared to the experimental group. The mean percentage of Ki-67 stained nuclei per 500 cells in the vaginal epithelium was 68.64±2.64 and 30.46±2.00 [mean±standard error of the mean (SEM)] in the control and experimental groups, respectively (p<0.003). Conclusion: This study showed that anastrozole, at the dose and treatment duration selected, significantly decreased cell proliferation in the vaginal mucosa of the rats in persistent estrus.
Databáze:	MEDLINE
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