| Autor: |
Silva JN; Programa de Pós-graduação em Microbiologia Agrícola, Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia (UFRB), Rua Rui Barbosa, 710, Centro, CEP 44380-000, Cruz das Almas, BA, Brasil., Baliza MD; Complexo Multidisciplinar de Estudos e Pesquisa em Saúde, Centro de Ciências da Saúde - COMEPS, Universidade Federal do Recôncavo da Bahia (UFRB), Avenida Carlos Amaral, R. do Cajueiro, 1015, CEP 44570-000, Santo Antônio de Jesus, BA, Brasil., Freitas F; Complexo Multidisciplinar de Estudos e Pesquisa em Saúde, Centro de Ciências da Saúde - COMEPS, Universidade Federal do Recôncavo da Bahia (UFRB), Avenida Carlos Amaral, R. do Cajueiro, 1015, CEP 44570-000, Santo Antônio de Jesus, BA, Brasil., Cruz ES; Programa de Pós-graduação em Microbiologia Agrícola, Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia (UFRB), Rua Rui Barbosa, 710, Centro, CEP 44380-000, Cruz das Almas, BA, Brasil.; Complexo Multidisciplinar de Estudos e Pesquisa em Saúde, Centro de Ciências da Saúde - COMEPS, Universidade Federal do Recôncavo da Bahia (UFRB), Avenida Carlos Amaral, R. do Cajueiro, 1015, CEP 44570-000, Santo Antônio de Jesus, BA, Brasil., Camilo VMA; Complexo Multidisciplinar de Estudos e Pesquisa em Saúde, Centro de Ciências da Saúde - COMEPS, Universidade Federal do Recôncavo da Bahia (UFRB), Avenida Carlos Amaral, R. do Cajueiro, 1015, CEP 44570-000, Santo Antônio de Jesus, BA, Brasil., Silva IMM; Programa de Pós-graduação em Microbiologia Agrícola, Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia (UFRB), Rua Rui Barbosa, 710, Centro, CEP 44380-000, Cruz das Almas, BA, Brasil.; Complexo Multidisciplinar de Estudos e Pesquisa em Saúde, Centro de Ciências da Saúde - COMEPS, Universidade Federal do Recôncavo da Bahia (UFRB), Avenida Carlos Amaral, R. do Cajueiro, 1015, CEP 44570-000, Santo Antônio de Jesus, BA, Brasil. |
| Abstrakt: |
Many Solidarity Economic Venture (SEV) are family farmers who seek to add value to production through artisanal processing, which can lead to food contamination. Thus, this study aimed to genotypically characterize thermotolerant coliforms (TtC) strains from food produced by local agribusinesses of SEV during January to April 2019. Samples from thirteen production units (PU) from the SEV were submitted to a microbiological analysis of thermotolerant coliforms (AFNOR 3M1/2 - 09/89), using a fast count method in Petrifilm™ dishes. The Polymerase Chain Reaction (PCR) technique was used to verify the following virulence genes (VGs) associated with Escherichia coli: stx, typical from enterohemorrhagic E. coli (EHEC); bfpA typical from entheropathogenic E. coli (EPEC) and elt and slt, typical from entherotoxigenic E. coli (ETEC). The results showed that two samples of queijadinha (typical Brazilian candy made with eggs and coconut) and one sample of cassava cake presented characteristic colonies TtC. This way, three strains were isolated in order to perform the PCR technique. However, the genes used in the reaction were not detected in the isolated strains. Therefore, it is suggested that the isolated strains are from E. coli pathotypes with different virulence genes than the ones analyzed belong other types of TtC, such as Enterobacter and Klebsiella. Although the virulence of genes has not been confirmed, the presence of TtC on food indicates hygiene flaws during production and, therefore, measurements to control and prevent contamination should be taken. |
