| Autor: |
Rodda AE; Department of Materials Science and Engineering, and Monash Institute for Medical Engineering, Monash University, Wellington Rd, Clayton 3800, Victoria, Australia. andrew.rodda@monash.edu john.forsythe@monash.edu., Ercole F, Glattauer V, Nisbet DR, Healy KE, Dove AP, Meagher L, Forsythe JS |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of materials chemistry. B [J Mater Chem B] 2016 Dec 07; Vol. 4 (45), pp. 7314-7322. Date of Electronic Publication: 2016 Nov 03. |
| DOI: |
10.1039/c6tb02444k |
| Abstrakt: |
While polycaprolactone (PCL) and similar polyesters are commonly used as degradable scaffold materials in tissue engineering and related applications, non-specific adsorption of environmental proteins typically precludes any control over the signalling pathways that are activated during cell adhesion to these materials. Here we describe the preparation of PCL-based fibres that facilitate cell adhesion through well-defined pathways while preventing adhesion via adsorbed proteins. Surface-initiated atom transfer radical polymerisation (SI-ATRP) was used to graft a protein-resistant polymer brush coating from the surface of fibres, which had been electrospun from a brominated PCL macroinitiator. This coating also provided alkyne functional groups for the attachment of specific signalling molecules via the copper-mediated azide-alkyne click reaction; in this case, a cyclic RGD peptide with high affinity for α v β 3 integrins. Mesenchymal stem cells were shown to attach to the fibres via the peptide, but did not attach in its absence, nor when blocked with soluble peptide, demonstrating the effective control of cell adhesion pathways. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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