| Autor: |
Jampaiah D; Centre for Advanced Materials & Industrial Chemistry (CAMIC), School of Applied Sciences, RMIT University, GPO BOX 2476, Melbourne-3001, Australia. suresh.bhargava@rmit.edu.in., Srinivasa Reddy T, Kandjani AE, Selvakannan PR, Sabri YM, Coyle VE, Shukla R, Bhargava SK |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of materials chemistry. B [J Mater Chem B] 2016 Jun 14; Vol. 4 (22), pp. 3874-3885. Date of Electronic Publication: 2016 May 12. |
| DOI: |
10.1039/c6tb00422a |
| Abstrakt: |
The construction of highly efficient inorganic mimetic enzymes (nanozymes) is much needed to replace natural enzymes due to their instability and high cost. Recently, nanoscale CeO 2 has been attracting significant interest due to its unique properties such as facile redox behaviour (Ce 4+ ↔ Ce 3+ ) and surface defects. In the present work, various amounts of Fe 3+ -doped CeO 2 nanorods (NRs) (with 3, 6, 9, and 12% Fe doping) were synthesized using a facile hydrothermal method and investigated for peroxidase-like activity and glucose detection. The peroxidase-like activity results revealed that 6 at% doping is the optimal Fe doping level to demonstrate superior catalytic performance over un-doped and Fe 3+ -doped CeO 2 NRs. Steady state kinetic analysis also confirms that the 6% Fe 3+ -doped CeO 2 (6Fe/CeO 2 ) NRs exhibited excellent catalytic performance towards 3,3',5,5'tetramethylbenzidine (TMB) oxidation with a K m and V m of 0.176 mM and 8.6 × 10 -8 M s -1 , respectively, as compared to horseradish peroxidase (HRP) enzymes (0.434 mM and 10.0 × 10 -8 M s -1 ). Typical colour reactions arising from the catalytic oxidation of the TMB substrate over 6Fe/CeO 2 NRs with H 2 O 2 have been utilized to establish a simple sensitive and selective colorimetric assay for the determination of glucose concentration in buffer, diluted fruit juices and foetal bovine serum samples. The superior catalytic performance of 6Fe/CeO 2 NRs could be attributed to abundant surface defects, high surface area and pore volume, and preferential exposure of the highly reactive (110) planes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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