A transmission electron microscopy study of anticoagulant-induced platelet vesiculation.
| Autor: | Almhanawi BH; Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia., Khalid B; Department of Medicine, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia., Ibrahim TA; Electron Microscopy Unit, Institute of Biomedical Sciences (IBS), University Putra Malaysia, Malaysia., Tohit ERM; Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia. |
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| Jazyk: | angličtina |
| Zdroj: | Porto biomedical journal [Porto Biomed J] 2017 Jan-Feb; Vol. 2 (1), pp. 23-27. Date of Electronic Publication: 2016 Dec 27. |
| DOI: | 10.1016/j.pbj.2016.11.002 |
| Abstrakt: | Highlights: EDTA induces platelet microparticles (PMPs) vesiculation.Sodium citrated tubes are better than EDTA tubes in PMPs analysis.Sodium citrate has a minor impact on platelet structure. Abstract: Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation. Competing Interests: The authors declare no conflicts of interest. (Copyright 2016 PBJ-Associação Porto Biomedical/Porto Biomedical Society.) |
| Databáze: | MEDLINE |
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