The large repertoire of 2'-O-methylation guided by C/D snoRNAs on Trypanosoma brucei rRNA.

Autor:	Rajan KS; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University , Ramat-Gan, Israel., Zhu Y; Department of Genetics and Genome Sciences, UConn Health , Farmington, CT, USA., Adler K; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University , Ramat-Gan, Israel., Doniger T; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University , Ramat-Gan, Israel., Cohen-Chalamish S; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University , Ramat-Gan, Israel., Srivastava A; Department of Genetics and Genome Sciences, UConn Health , Farmington, CT, USA., Shalev-Benami M; Department of Structural Biology, Weizmann Institute of Science , Rehovot, Israel., Matzov D; Department of Structural Biology, Weizmann Institute of Science , Rehovot, Israel., Unger R; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University , Ramat-Gan, Israel., Tschudi C; Departmentof Epidemiology and Microbial Diseases, Yale School of Public Health , New Haven, CT, USA., Günzl A; Department of Genetics and Genome Sciences, UConn Health , Farmington, CT, USA., Carmichael GG; Department of Genetics and Genome Sciences, UConn Health , Farmington, CT, USA., Michaeli S; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University , Ramat-Gan, Israel.
Jazyk:	angličtina
Zdroj:	RNA biology [RNA Biol] 2020 Jul; Vol. 17 (7), pp. 1018-1039. Date of Electronic Publication: 2020 Apr 21.
DOI:	10.1080/15476286.2020.1750842
Abstrakt:	The parasite Trypanosoma brucei cycles between insect and mammalian hosts, and is the causative agent of sleeping sickness. Here, we performed genome-wide mapping of 2'- O -methylations (Nms) on trypanosome rRNA using three high-throughput sequencing methods; RibOxi-seq, RiboMeth-seq and 2'- O Me-seq. This is the first study using three genome-wide mapping approaches on rRNA from the same species showing the discrepancy among the methods. RibOxi-seq detects all the sites, but RiboMeth-seq is the only method to evaluate the level of a single Nm site. The sequencing revealed at least ninety-nine Nms guided by eighty-five snoRNAs among these thirty-eight Nms are trypanosome specific sites. We present the sequence and target of the C/D snoRNAs guiding on rRNA. This is the highest number of Nms detected to date on rRNA of a single cell parasite. Based on RiboMeth-seq, several Nm sites were found to be differentially regulated at the two stages of the parasite life cycle, the insect procyclic form (PCF) versus the bloodstream form (BSF) in the mammalian host.
Databáze:	MEDLINE
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