| Autor: |
Amarillas L; Instituto de Investigación Lightbourn, A. C., Carretera Las Pampas Km. 2.5, 33980, Ciudad Jiménez, Chihuahua, México., Estrada-Acosta M; Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México., León-Chan RG; Instituto de Investigación Lightbourn, A. C., Carretera Las Pampas Km. 2.5, 33980, Ciudad Jiménez, Chihuahua, México., López-Orona C; Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México., Lightbourn L; Instituto de Investigación Lightbourn, A. C., Carretera Las Pampas Km. 2.5, 33980, Ciudad Jiménez, Chihuahua, México. drlightbourn@institutolightbourn.edu.mx. |
| Abstrakt: |
The GenBank database contains over 2580 complete genome sequences from bacteriophages. However, limited reports are available concerning phages can that lyse members of Pseudomonas syringae, although this is a widespread bacterial species that can infect almost 200 plant species. In the present study, we isolated and characterized a new Siphoviridae phage, named "Pseudomonas phage vB_PsyS_Phobos" (for brevity, referred to here as Phobos). To our knowledge, this is one of the first genome sequences reported for a phage with lytic activity against P. syringae pv. syringae. The genome of Phobos is dsDNA of 56,734 bp with a GC content of 63.3%, containing 65 ORFs. Genome analysis revealed that Phobos is a novel lytic phage with unique genomic features and low similarity to other phages, suggesting that Phobos represents a new phage genus. Genome sequencing did not reveal sequences with significant similarity to known virulence factors, antibiotic resistance genes, potential immunoreactive allergens, or lysogeny-related proteins, suggesting suggests that phage Phobos is strictly lytic. Therefore, Phobos may be suitable for formulation as a biocontrol agent against P. syringae pv. syringae. |
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