Root-knot nematodes modulate cell walls during root-knot formation in Arabidopsis roots.

Autor: Ishida T; International Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, Kurokami 2-39-1, Kumamoto, 860-8555, Japan. ishida-takashi@kumamoto-u.ac.jp., Suzuki R; Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan., Nakagami S; Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan., Kuroha T; Graduate School of Life Sciences, Tohoku University, Sendai, Japan.; Division of Applied Genetics, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan., Sakamoto S; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan., Nakata MT; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan., Yokoyama R; Graduate School of Life Sciences, Tohoku University, Sendai, Japan., Kimura S; Department of Industrial Life Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.; Center for Ecological Evolutionary Developmental Biology, Kyoto Sangyo University, Kyoto, Japan., Mitsuda N; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan., Nishitani K; Graduate School of Life Sciences, Tohoku University, Sendai, Japan., Sawa S; Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
Jazyk: angličtina
Zdroj: Journal of plant research [J Plant Res] 2020 May; Vol. 133 (3), pp. 419-428. Date of Electronic Publication: 2020 Apr 03.
DOI: 10.1007/s10265-020-01186-z
Abstrakt: Phytoparasitic nematodes parasitize many species of rooting plants to take up nutrients, thus causing severe growth defects in the host plants. During infection, root-knot nematodes induce the formation of a characteristic hyperplastic structure called a root-knot or gall on the roots of host plants. Although many previous studies addressed this abnormal morphogenesis, the underlying mechanisms remain uncharacterized. To analyze the plant-microorganism interaction at the molecular level, we established an in vitro infection assay system using the nematode Meloidogyne incognita and the model plant Arabidopsis thaliana. Time-course mRNA-seq analyses indicated the increased levels of procambium-associated genes in the galls, suggesting that vascular stem cells play important roles in the gall formation. Conversely, genes involved in the formation of secondary cell walls were decreased in galls. A neutral sugar analysis indicated that the level of xylan, which is one of the major secondary cell wall components, was dramatically reduced in the galls. These observations were consistent with the hypothesis of a decrease in the number of highly differentiated cells and an increase in the density of undifferentiated cells lead to gall formation. Our findings suggest that phytoparasitic nematodes modulate the developmental mechanisms of the host to modify various aspects of plant physiological processes and establish a feeding site.
Databáze: MEDLINE
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