| Autor: |
Peixoto JVC; Department of Physiology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., Santos ASR Jr; Department of Physiology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., Corso CR; Department of Pharmacology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., da Silva FS; Department of Health Sciences, Federal Rural University of the Semi-Arid, Av. Francisco Mota 572, Pres. Costa e Silva, Mossoró, Rio Grande do Norte 59625-900, Brazil., Capote A; Department of Physiology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., Ribeiro CD; Department of Physiology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., Abreu BJDGA; Department of Morphology, Federal University of Rio Grande do Norte, Av. Senador Salgado Filho 3000, Candelária, Natal, Rio Grande do Norte 59064-741, Brazil., Acco A; Department of Pharmacology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., Fogaça RH; Department of Physiology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil., Dias FAL; Department of Physiology, Federal University of Paraná, Av. Francisco H. dos Santos 100, Jardim das Américas, Curitiba, Paraná 81531-980, Brazil. |
| Abstrakt: |
Diabetes mellitus is a metabolic disorder that can generate tissue damage through several pathways. Alteration and dysfunction of skeletal muscle are reported including respiratory muscles, which may compromise respiratory parameters in diabetic patients. We have aimed to evaluate the diaphragm muscle contractility, tissue remodeling, oxidative stress, and inflammatory parameters from 30 day streptozotocin-treated rats. The diaphragm contractility was assessed using isolated muscle, tissue remodeling using histology and zymography techniques, and tissue oxidative stress and inflammatory parameters by enzyme activity assay. Our data revealed in the diabetes mellitus group an increase in maximum tetanic force (4.82 ± 0.13 versus 4.24 ± 0.18 N/cm 2 ( p = 0.015)) and fatigue resistance (139.16 ± 10.78 versus 62.25 ± 4.45 s ( p < 0.001)), reduction of 35.4% in muscle trophism ( p < 0.001), increase of 32.6% of collagen deposition ( p = 0.007), reduction of 21.3% in N -acetylglucosaminidase activity ( p < 0.001), and increase of 246.7% of catalase activity ( p = 0.002) without changes in reactive oxygen species ( p = 0.518) and tissue lipid peroxidation ( p = 0.664). All observed changes are attributed to the poor glycemic control (471.20 ± 16.91 versus 80.00 ± 3.42 mg/dL ( p < 0.001)), which caused defective tissue regeneration and increased catalase activity as a compensatory mechanism. |
