Characterization and effect of nanocomplexed fluoride solutions on the inhibition of enamel demineralization created by a multispecies cariogenic biofilm model.

Autor: Vieira TI; Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Alexandria AK; Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Menezes JCV; School of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., do Amaral LH; School of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Dos Santos TMP; Laboratory for Nuclear Instrumentation, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Neves AA; Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.; Clinical Lecturer in Paediatric Dentistry, King's College London, London, UK., Lopes RT; Laboratory for Nuclear Instrumentation, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Cabral LM; School of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Valença AMG; Department of Clinic and Social Dentistry, School of Dentistry, Universidade Federal da Paraíba, Joao Pessoa, Paraíba, Brazil., Maia LC; Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. rorefa@terra.com.br.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2020 Nov; Vol. 24 (11), pp. 3947-3959. Date of Electronic Publication: 2020 Mar 31.
DOI: 10.1007/s00784-020-03261-0
Abstrakt: Objectives: The aim of this study was to assess the in vitro caries preventive effect of nanocomplexed solutions of hydroxypropyl-β-cyclodextrin and γ-cyclodextrin associated with titanium tetrafluoride (TiF 4 ) after different complexation times (12 or 72 h).
Materials and Methods: Enamel blocks were randomly distributed in 9 groups (n = 11): negative control, hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, TiF 4 , hydroxypropyl-β-cyclodextrin:TiF 4 12 h, hydroxypropyl-β-cyclodextrin:TiF 4 72 h, γ-cyclodextrin:TiF 4 12 h, γ-cyclodextrin:TiF 4 72 h, and NaF (positive control). The solutions were applied for 1 min and the blocks were exposed to a biofilm model. Nanocompounds were characterized by differential scanning calorimetry and X-ray powder diffraction. The percentage of surface microhardness loss (%SML), mineral density changes (ΔZ), lesion depth, surface morphology (scanning electron microscopy-SEM), and chemical characterization (energy-dispersive spectroscopy-EDS) were assessed.
Results: No oxidation was observed, and the formation of the nanocomplexes was evidenced by changes in the melting point compared to pure cyclodextrins and the loss of crystallinity of the materials. Hydroxypropyl-β-cyclodextrin:TiF 4 72 h resulted in lower %SML than negative control, hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, and TiF 4 (p < 0.05). NaF differed from all groups (p < 0.05), except for hydroxypropyl-β-cyclodextrin:TiF 4 72 h (p = 0.83). ΔZ of hydroxypropyl-β-cyclodextrin:TiF 4 72 h was higher than negative control, hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, γ-cyclodextrin:TiF 4 1 2 h, γ-cyclodextrin:TiF 4 72 h, and NaF (p < 0.05) and similar to TiF 4 and hydroxypropyl-β-cyclodextrin:TiF 4 12 h (p > 0.05). SEM/EDS detected Ti in the blocks subjected to TiF 4 -products.
Conclusion: The hydroxypropyl-β-cyclodextrin:TiF 4 72 h solution showed caries preventive effect on the surface and subsurface of the enamel.
Clinical Relevance: A hydroxypropyl-β-cyclodextrin nanosystem, in association with TiF 4 after 72 h of complexation, may be a promising agent for the prevention of enamel demineralization.
Databáze: MEDLINE
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