Long non-coding RNA CRNDE deteriorates intrauterine infection-induced neonatal brain injury.

Autor: Fu CH; Department of Neonatology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China., Zhang BH; Department of Neonatology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China., Fang CZ; Department of Neonatology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China., Yan CX; Department of Neonatology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China., Lai FF; Department of Pediatric, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China., Chen S; Department of Neonatology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China., Wang GH; Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China. Electronic address: wgaohua_23@163.com.
Jazyk: angličtina
Zdroj: Molecular and cellular probes [Mol Cell Probes] 2020 Aug; Vol. 52, pp. 101565. Date of Electronic Publication: 2020 Mar 29.
DOI: 10.1016/j.mcp.2020.101565
Abstrakt: Background: This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats.
Methods: Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia.
Results: LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS.
Conclusion: CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection.
Competing Interests: Declaration of competing interest All the authors declare no conflict of interest.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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