Dynamic change process of target genes by RT-PCR testing of SARS-Cov-2 during the course of a Coronavirus Disease 2019 patient.

Autor: Lv DF; Department of Blood Transfusion, Ningbo First Hospital, Ningbo, China; Department of Blood Transfusion, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China; Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Ying QM; Department of Blood Transfusion, Ningbo First Hospital, Ningbo, China; Department of Blood Transfusion, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Weng YS; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China; Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Shen CB; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China; Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Chu JG; Department of General Practice, Ningbo First Hospital, Ningbo, China; Department of General Practice, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Kong JP; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China; Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Sun DH; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China; Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Gao X; Department of Neurosurgery, Ningbo First Hospital, Ningbo, China; Department of Neurosurgery, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China., Weng XB; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China; Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China. Electronic address: wxb6006@hotmail.com., Chen XQ; Department of Chinese Traditional Medicine, Ningbo First Hospital, Ningbo, China; Department of Chinese Traditional Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China. Electronic address: cxq2316@163.com.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2020 Jul; Vol. 506, pp. 172-175. Date of Electronic Publication: 2020 Mar 27.
DOI: 10.1016/j.cca.2020.03.032
Abstrakt: We report the dynamic change process of target genes by RT-PCR testing of SARS-Cov-2 during the course of a COVID-19 patient: from successive negative results to successive single positive nucleocapsid gene, to two positive target genes (orf1ab and nucleocapsid) by RT-PCR testing of SARS-Cov-2, and describe the diagnosis, clinical course, and management of the case. In this case, negative results of RT-PCR testing was not excluded to diagnose a suspected COVID-19 patient, clinical signs and symptoms, other laboratory findings, and chest CT images should be taken into account for the absence of enough positive evidence. This case highlights the importance of successive sampling and testing SARS-Cov-2 by RT-PCR as well as the increased value of single positive target gene from pending to positive in two specimens to diagnose laboratory-confirmed COVID-19.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest. We obtained the approval letter of Ningbo First Hospital Ethics Committee (Approval No. 2020-R037).
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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