Gold-microrods/Pd-nanoparticles/polyaniline-nanocomposite-interface as a peroxidase-mimic for sensitive detection of tropomyosin.

Autor: Mohamad A; Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410, Brunei Darussalam., Rizwan M; Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410, Brunei Darussalam; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, Wales, United Kingdom., Keasberry NA; Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410, Brunei Darussalam., Nguyen AS; Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Viet Nam., Lam TD; Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Viet Nam., Ahmed MU; Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410, Brunei Darussalam. Electronic address: minhaz.ahmed@ubd.edu.bn.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2020 May 01; Vol. 155, pp. 112108. Date of Electronic Publication: 2020 Feb 19.
DOI: 10.1016/j.bios.2020.112108
Abstrakt: In this study, Gold-microrods (AuMRs), Pd-nanoparticles (PdNPs), and Polyaniline (PANI) nanocomposite-interface was fabricated on the screen-printed carbon-microelectrode (SPE). Each layer of the interface was characterised using field emission-scanning electron microscopy (FE-SEM) and cyclic voltammetry (CV). The fabricated SPE/AuMRs/PdNPs/PANI interface demonstrated the highest electronic current and showed excellent peroxidase-mimic towards H 2 O 2 using chronoamperometry (CA). Furthermore, the SPE/AuMRs/PdNPs/PANI interface was utilised for the construction of a highly sensitive label-free electrochemical biosensor for the detection of Tpm in seafood samples. Label-free electrochemical detection of the Tpm was performed using both CA and differential pulse voltammetry (DPV) techniques. Preliminary data showed that both methods could detect Tpm as low as 0.01 pg/mL. Moreover, the developed biosensor for the detection of Tpm demonstrated excellent selectivity, high reproducibility and longer stability with an evident potential to detect Tpm in real seafood samples.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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