Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice.

Autor: Rodrigues MS; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Pieri BLDS; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Silveira GB; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Zaccaron RP; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Venturini LM; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Comin VH; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Luiz KD; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil., Silveira PCL; Universidade do Extremo Sul Catarinense , Criciúma , SC , Brazil.
Jazyk: English; Portuguese
Zdroj: Einstein (Sao Paulo, Brazil) [Einstein (Sao Paulo)] 2020 Mar 23; Vol. 18, pp. eAO5022. Date of Electronic Publication: 2020 Mar 23 (Print Publication: 2020).
DOI: 10.31744/einstein_journal/2020AO5022
Abstrakt: Objective: To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice.
Methods: Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage.
Results: Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group.
Conclusion: Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them.
Databáze: MEDLINE
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