Detection of 793/B serotype of infectious bronchitis virus in tissue sample by indirect immunoperoxidase assay.

Autor: Shamsaddini-Bafti M; 1Razi Vaccine & Serum Research Institute, Kerman Branch, km. 17th of Joupar, Kerman Rd, Kerman, Iran Post. Box: 76175-359., Vasfi-Marandi M; 2Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Momayez R; 3Razi Vaccine & Serum Research Institute, Karaj, Iran., Toroghi R; 3Razi Vaccine & Serum Research Institute, Karaj, Iran., Pourbakhsh SA; 3Razi Vaccine & Serum Research Institute, Karaj, Iran., Salari R; 4Faculty of Sciences, University of Tehran, Tehran, Iran., Tabrizchi H; Faculty of Medicine, University of Kerman, Kerman, Iran.
Jazyk: angličtina
Zdroj: Comparative clinical pathology [Comp Clin Path] 2014; Vol. 23 (2), pp. 347-352. Date of Electronic Publication: 2012 Sep 29.
DOI: 10.1007/s00580-012-1621-4
Abstrakt: The indirect immunoperoxidase (IIP) assay was compared with the reverse transcription-polymerase chain reaction (RT-PCR) for detection of 793/B serotype of infectious bronchitis virus in tissues samples collected from experimentally infected chickens. This technique was optimized in specific pathogen-free (SPF)-embryonated chicken eggs and broiler chickens inoculated with the Iranian IR/773/2001 strain of 793/B serotype The trachea, lung, kidney, and cecal tonsil tissue samples from experimentally infected chicken embryos and chickens were collected in order to prepare tissue sections in IIP assay and to detect in RT-PCR. The sensitivity and specificity values of IIP assay were, respectively, 83 and 84 %, and the positive and negative prediction values were 71 and 91 % when compared with RT-PCR.
(© Springer-Verlag London 2012.)
Databáze: MEDLINE
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