c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae.

Autor: Floyd KA; Department of Microbiology and Environmental Toxicology, University of California - Santa Cruz, 1156 High St., BioMed 245, Santa Cruz, CA, 95064, USA., Lee CK; Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California - Los Angeles, 420 Westwood Plaza, Room 5121 Engineering V, Los Angeles, CA, 90095, USA., Xian W; Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California - Los Angeles, 420 Westwood Plaza, Room 5121 Engineering V, Los Angeles, CA, 90095, USA., Nametalla M; Department of Biology, Brooklyn College, Room 307NE, 2900 Bedford Ave., Brooklyn, NY, 11210, USA.; CUNY Graduate Center, 365 5th Ave., New York, NY, 10016, USA., Valentine A; Department of Biology, Brooklyn College, Room 307NE, 2900 Bedford Ave., Brooklyn, NY, 11210, USA.; CUNY Graduate Center, 365 5th Ave., New York, NY, 10016, USA., Crair B; Department of Microbial Pathogenesis, Yale University, 840 West Campus Drive, Advanced Biosciences Center 211, West Haven, CT, 06516, USA., Zhu S; Department of Microbial Pathogenesis, Yale University, 840 West Campus Drive, Advanced Biosciences Center 211, West Haven, CT, 06516, USA., Hughes HQ; Department of Biology, Indiana University - Bloomington, 1001 East Third St., Jordan Hall 469A, Bloomington, IN, 47405, USA., Chlebek JL; Department of Biology, Indiana University - Bloomington, 1001 East Third St., Jordan Hall 469A, Bloomington, IN, 47405, USA., Wu DC; Department of Microbiology and Environmental Toxicology, University of California - Santa Cruz, 1156 High St., BioMed 245, Santa Cruz, CA, 95064, USA., Hwan Park J; Department of Microbiology and Environmental Toxicology, University of California - Santa Cruz, 1156 High St., BioMed 245, Santa Cruz, CA, 95064, USA., Farhat AM; Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California - Los Angeles, 420 Westwood Plaza, Room 5121 Engineering V, Los Angeles, CA, 90095, USA., Lomba CJ; Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California - Los Angeles, 420 Westwood Plaza, Room 5121 Engineering V, Los Angeles, CA, 90095, USA., Ellison CK; Department of Biology, Indiana University - Bloomington, 1001 East Third St., Jordan Hall 469A, Bloomington, IN, 47405, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, 355 Thomas Laboratory, Washington Road, Princeton, NJ, 08544, USA., Brun YV; Department of Microbiology, Infectious Diseases, and Immunology, Faculty of Medicine, University of Montreal, Pavillon Roger-Gaudry, 2900, boulevard Édouard-Montpetit, C.P. 6128, Succursale Centre-ville, Montréal, QC, H3C 3J7, Canada., Campos-Gomez J; Cystic Fibrosis Research Center, University of Alabama at Birmingham, 1918 University Blvd., MCLM 702, Birmingham, AL, 35233, USA., Dalia AB; Department of Biology, Indiana University - Bloomington, 1001 East Third St., Jordan Hall 469A, Bloomington, IN, 47405, USA., Liu J; Department of Microbial Pathogenesis, Yale University, 840 West Campus Drive, Advanced Biosciences Center 211, West Haven, CT, 06516, USA., Biais N; Department of Biology, Brooklyn College, Room 307NE, 2900 Bedford Ave., Brooklyn, NY, 11210, USA.; CUNY Graduate Center, 365 5th Ave., New York, NY, 10016, USA., Wong GCL; Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California - Los Angeles, 420 Westwood Plaza, Room 5121 Engineering V, Los Angeles, CA, 90095, USA. gclwong@seas.ucla.edu., Yildiz FH; Department of Microbiology and Environmental Toxicology, University of California - Santa Cruz, 1156 High St., BioMed 245, Santa Cruz, CA, 95064, USA. fyildiz@ucsc.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2020 Mar 25; Vol. 11 (1), pp. 1549. Date of Electronic Publication: 2020 Mar 25.
DOI: 10.1038/s41467-020-15331-8
Abstrakt: Biofilm formation by Vibrio cholerae facilitates environmental persistence, and hyperinfectivity within the host. Biofilm formation is regulated by 3',5'-cyclic diguanylate (c-di-GMP) and requires production of the type IV mannose-sensitive hemagglutinin (MSHA) pilus. Here, we show that the MSHA pilus is a dynamic extendable and retractable system, and its activity is directly controlled by c-di-GMP. The interaction between c-di-GMP and the ATPase MshE promotes pilus extension, whereas low levels of c-di-GMP correlate with enhanced retraction. Loss of retraction facilitated by the ATPase PilT increases near-surface roaming motility, and impairs initial surface attachment. However, prolonged retraction upon surface attachment results in reduced MSHA-mediated surface anchoring and increased levels of detachment. Our results indicate that c-di-GMP directly controls MshE activity, thus regulating MSHA pilus extension and retraction dynamics, and modulating V. cholerae surface attachment and colonization.
Databáze: MEDLINE