| Autor: |
de Souza Ferreira Bringel PH; Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, 60714-903, Brazil., Marques GFO; Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, 60714-903, Brazil., de Queiroz Martins MG; Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, 60455-970, Brazil., da Silva MTL; Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, 60455-970, Brazil., Nobre CAS; Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, 60455-970, Brazil., do Nascimento KS; Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, 60455-970, Brazil., Cavada BS; Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, 60455-970, Brazil., Castro RR; Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, 60714-903, Brazil., Assreuy AMS; Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, 60714-903, Brazil. anassreuy@gmail.com. |
| Abstrakt: |
This study investigated the effects of the alga lectin Hypnea cervicornis agglutinin (HCA) on rat zymosan-induced arthritis (ZyA). Zymosan (50-500 μg/25 μL) or sterile saline (Sham) was injected into the tibio-tarsal joint of female Wistar rats (180-200 g). Arthritic animals received morphine (4 mg/kg, intraperitoneal), indomethacin (5 mg/kg, intraperitoneal), or 2% lidocaine (100 μL, subcutaneous). HCA (0.3-3 mg/kg) was administered by intravenous route 30 min before or 2 h after zymosan. 1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one (ODQ, 4 μg, intra-articular) was given 30 min prior HCA. Hypernociception was measured every hour until 6 h, time in which animals were sacrificed for evaluation of leukocytes of the intra articular fluid and gene expression of TNF-α, IL-1, IL-10, and iNOS in the joint tissues using PCR techniques. Hypernociception was responsive to morphine and indomethacin, and its threshold was not altered by lidocaine. The post-treatment of HCA reduced both hypernociception and leukocyte influx. This antinociceptive effect was abolished either by ODQ and glibenclamide. HCA also reduced gene expression of iNOS and TNF-α. In conclusion, the antinociceptive effect of HCA in ZyA involves cyclic GMP signalization and selective modulation of cytokine expression. |
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