Sequential degradation of raw vinasse by a laccase enzyme producing fungus Pleurotus sajor-caju and its ATPS purification.

Autor: Junior JA; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil., Vieira YA; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil., Cruz IA; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil., da Silva Vilar D; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil., Aguiar MM; Division of Molecular Biology - Biocenter, Innsbruck Medical University, A-6020, Innsbruck, Austria., Torres NH; Institute of Technology and Research, Av. Murilo Dantas 300 - Prédio do ITP, Farolândia, 49032-490, Aracaju, SE, Brazil., Bharagava RN; Laboratory for Bioremediation and Metagenomics Research (LBMR), Department of Microbiology (DM), Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, 226 025, Uttar Pradesh, India., Lima ÁS; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil.; Institute of Technology and Research, Av. Murilo Dantas 300 - Prédio do ITP, Farolândia, 49032-490, Aracaju, SE, Brazil., de Souza RL; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil.; Institute of Technology and Research, Av. Murilo Dantas 300 - Prédio do ITP, Farolândia, 49032-490, Aracaju, SE, Brazil., Romanholo Ferreira LF; Graduate Program in Process Engineering, Tiradentes University, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil.; Institute of Technology and Research, Av. Murilo Dantas 300 - Prédio do ITP, Farolândia, 49032-490, Aracaju, SE, Brazil.
Jazyk: angličtina
Zdroj: Biotechnology reports (Amsterdam, Netherlands) [Biotechnol Rep (Amst)] 2019 Dec 13; Vol. 25, pp. e00411. Date of Electronic Publication: 2019 Dec 13 (Print Publication: 2020).
DOI: 10.1016/j.btre.2019.e00411
Abstrakt: This study evaluated simultaneously the raw vinasse degradation, an effluent from the sugar-alcohol industry, the laccase production by Pleurotus sajor-caju and its purification using aqueous two-phase systems (ATPS). To improve laccase production, different concentrations of inducers (ethanol and CuSO 4 ) were added. The higher laccase production promoted an increase of 4-fold using 0.4 mM of CuSO 4 as inducer, with maximum enzymatic activity of 539.3 U/L on the 3 rd day of fermentation. The final treated vinasse had a decolorization of 92% and turbidity removal of 99% using CuSO 4 . Moreover, the produced laccase was then purified by ATPS in a single purification step, reaching 2.9-fold and recovered ≈ 99,9 %, in the top phase (PEG-rich phase) using 12 wt% of PEG 1500 + 20 wt% of citrate buffer + enzyme broth + water, at 25 °C. Thus, an integrated process of vinasse degradation, laccase production and purification with potential industrial application was proposed.
(© 2019 The Authors.)
Databáze: MEDLINE
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