Dried Blood Spot Tests for the Diagnosis and Therapeutic Monitoring of HIV and Viral Hepatitis B and C.

Autor:	Tuaillon E; Pathogenèse et Contrôle des Infections Chroniques, INSERM U1058, Centre Hospitalier Universitaire de Montpellier, Montpellier, France., Kania D; Centre Muraz, Bobo Dioulasso, Burkina Faso., Pisoni A; Pathogenèse et Contrôle des Infections Chroniques, INSERM U1058, Centre Hospitalier Universitaire de Montpellier, Montpellier, France., Bollore K; Pathogenèse et Contrôle des Infections Chroniques, INSERM U1058, Centre Hospitalier Universitaire de Montpellier, Montpellier, France., Taieb F; Emerging Diseases Epidemiology Unit, Center for Translational Science, Institut Pasteur, Paris, France., Ontsira Ngoyi EN; Pathogenèse et Contrôle des Infections Chroniques, INSERM U1058, Centre Hospitalier Universitaire de Montpellier, Montpellier, France., Schaub R; CIC AG/INSERM 1424, Centre Hospitalier de Cayenne, Cayenne, France., Plantier JC; Laboratoire de Virologie, Normandie Université, CHU de Rouen, Rouen, France., Makinson A; INSERM U1175/IRD UMI 233, IRD, CHU de Montpellier, Montpellier, France., Van de Perre P; Pathogenèse et Contrôle des Infections Chroniques, INSERM U1058, Centre Hospitalier Universitaire de Montpellier, Montpellier, France.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2020 Mar 09; Vol. 11, pp. 373. Date of Electronic Publication: 2020 Mar 09 (Print Publication: 2020).
DOI:	10.3389/fmicb.2020.00373
Abstrakt:	Blood collected and dried on a paper card - dried blood spot (DBS) - knows a growing interest as a sampling method that can be performed outside care facilities by capillary puncture, and transported in a simple and safe manner by mail. The benefits of this method for blood collection and transport has recently led the World Health Organization to recommend DBS for HIV and hepatitis B and C diagnosis. The clinical utility of DBS sampling to improve diagnostics and care of HIV and hepatitis B and C infection in hard to reach populations, key populations and people living in low-income settings was highlighted. Literature about usefulness of DBS specimens in the therapeutic cascade of care - screening, confirmation, quantification of nucleic acids, and resistance genotyping -, was reviewed. DBS samples are suitable for testing antibodies, antigens, or nucleic acids using most laboratory methods. Good sensibility and specificity have been reported for infant HIV diagnosis and diagnosis of hepatitis B and C. The performance of HIV RNA testing on DBS to identified virological failure on antiretroviral therapy is also high but not optimal because of the dilution of dried blood in the elution buffer, reducing the analytical sensitivity, and because of the contamination by intracellular HIV DNA. Standardized protocols are needed for inter-laboratory comparisons, and manufacturers should pursue regulatory approval for in vitro diagnostics using DBS specimens. Despite these limitations, DBS sampling is a clinically relevant tool to improve access to infectious disease diagnosis worldwide. (Copyright © 2020 Tuaillon, Kania, Pisoni, Bollore, Taieb, Ontsira Ngoyi, Schaub, Plantier, Makinson and Van de Perre.)
Databáze:	MEDLINE
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