Fast pH-mediated changes of the viscosity of protein solutions studied with a voltage-modulated quartz crystal microbalance.

Autor: Gödde A; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany., Leppin C; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany., Meyer FS; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany., Langhoff A; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany., Hartl J; Boehringer Ingelheim Pharma GmbH & Co. KG, Innovation Unit, PDB, Birkendorfer Str. 65, D-88397 Biberach/Riss, Germany., Garidel P; Boehringer Ingelheim Pharma GmbH & Co. KG, Innovation Unit, PDB, Birkendorfer Str. 65, D-88397 Biberach/Riss, Germany., Johannsmann D; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany.
Jazyk: angličtina
Zdroj: Biointerphases [Biointerphases] 2020 Mar 24; Vol. 15 (2), pp. 021004. Date of Electronic Publication: 2020 Mar 24.
DOI: 10.1116/1.5140619
Abstrakt: An electrochemical quartz crystal microbalance is described, which achieves a time resolution down to 100 μs. Accumulation and averaging over a few hours bring the noise down to about 30 mHz. The application examples are pH-driven viscosity changes in albumin solutions. The pH was switched with the electrode potential. The characteristic response time is in the millisecond range. The focus is on experimental aspects as well as advantages and limitations of the technique.
Databáze: MEDLINE
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