Tracking Longitudinal Rotation of Silicon Nanowires for Biointerfaces.
| Autor: | Lee YV; Department of Chemistry, University of Chicago, Chicago, Illinois 60637, United States., Wu D; Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago, Chicago, Illinois 60637, United States., Fang Y; Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago, Chicago, Illinois 60637, United States., Peng Y; Research Computing Center, University of Chicago, Chicago, Illinois 60637, United States., Tian B; Department of Chemistry, University of Chicago, Chicago, Illinois 60637, United States.; James Franck Institute, The University of Chicago, Chicago, Illinois 60637, United States. |
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| Jazyk: | angličtina |
| Zdroj: | Nano letters [Nano Lett] 2020 May 13; Vol. 20 (5), pp. 3852-3857. Date of Electronic Publication: 2020 Apr 05. |
| DOI: | 10.1021/acs.nanolett.0c00974 |
| Abstrakt: | The rolling motion (i.e., longitudinal rotation) of nanomaterials may serve as a proxy to probe microscopic environments. Furthermore, nanoscale rotations in biological systems are common but difficult to measure. Here, we report a new tool that measures rolling motion of a nanowire with a short arm grown at one end. We present a particle detection algorithm with subpixel resolution and image segmentation with principal component analysis that enables precise and automated determination of the nanowire orientation. We show that the nanowires' rolling dynamics can be significantly affected by their surroundings and demonstrate the probes' ability to reflect different nanobio interactions. A non-cell-interacting nanowire undergoes rapid subdiffusive rotation, while a cell-interacting nanowire exhibits superdiffusive unidirectional rotation when the cell membrane actively interacts with the nanowire and slow subdiffusive rotation when it is fully encompassed by the cell. Our method can be used to yield insights into various biophysical and assembly processes. |
| Databáze: | MEDLINE |
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