Evaluation of bone regeneration potential of injectable extracellular matrix (ECM) from porcine dermis loaded with biphasic calcium phosphate (BCP) powder.

Autor: Ventura RD; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Republic of Korea., Padalhin AR; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Republic of Korea., Kim B; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Republic of Korea., Park M; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Republic of Korea., Lee BT; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Republic of Korea; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Republic of Korea. Electronic address: lbt@sch.ac.kr.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2020 May; Vol. 110, pp. 110663. Date of Electronic Publication: 2020 Jan 16.
DOI: 10.1016/j.msec.2020.110663
Abstrakt: Extracellular matrix (ECM) contains a wide array of complex proteins, growth factors and cytokines that regulate cell behavior and tissue development. ECM harvested from non-homologous ECM sources still provide a structural support and biochemical cues to cells for effective tissue remodeling. The aim of this study is to evaluate the effect of non-tissue specific decellularized ECM from porcine dermis loaded with biphasic calcium phosphate powder (BCP) in bone regeneration. Thermosensitive ECM hydrogels with BCP powder exhibited a porous morphology with a suitable injectability and increased mechanical stability. In-vitro studies using MC3T3-E1 pre osteoblast cells showed that the injectable ECM hydrogels were biocompatible and supported the osteogenic differentiation. The bone regeneration capacity of the injectable ECM hydrogels was evaluated in-vivo by implanting in rat femoral head for 4 and 8 weeks. Micro-CT and histological staining results indicated that the injectable ECM hydrogels loaded with BCP powder showed higher and improved bone formation compared with the unfilled defect. Injectable ECM loaded with BCP powder is a good potential biomaterial for non-load bearing bone regeneration application.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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