Agaphelin modulates the activation of human bronchial epithelial cells induced by lipopolysaccharide and IL-4.

Autor: Favarin DC; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triângulo Mineiro, Uberaba, MG, 38025-350, Brazil. Electronic address: danielyfavarin@hotmail.com., Pereira ABM; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triângulo Mineiro, Uberaba, MG, 38025-350, Brazil. Electronic address: alinebmp@gmail.com., Francischetti IMB; Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA. Electronic address: ivofrancischetti@gmail.com., da Silva MV; Laboratory of Immunology and Infectious Diseases, Triângulo Mineiro Federal University, Uberaba, Brazil. Electronic address: marcosuftm@gmail.com., Rodrigues V Jr; Laboratory of Immunology and Infectious Diseases, Triângulo Mineiro Federal University, Uberaba, Brazil. Electronic address: virmondes.rodrigues@uftm.edu.br., da Silva PR; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triângulo Mineiro, Uberaba, MG, 38025-350, Brazil. Electronic address: pauloroberto@cefores.uftm.edu.br., Valenzuela JG; Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA. Electronic address: jvalenzuela@niaid.nih.gov., Teixeira DNS; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triângulo Mineiro, Uberaba, MG, 38025-350, Brazil. Electronic address: david.teixeira@uftm.edu.br., Oliveira CJF; Laboratory of Immunology and Infectious Diseases, Triângulo Mineiro Federal University, Uberaba, Brazil. Electronic address: carlo.oliveira@uftm.edu.br., Rogério AP; Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triângulo Mineiro, Uberaba, MG, 38025-350, Brazil. Electronic address: alexandre.rogerio@uftm.edu.br.
Jazyk: angličtina
Zdroj: Immunobiology [Immunobiology] 2020 May; Vol. 225 (3), pp. 151937. Date of Electronic Publication: 2020 Mar 18.
DOI: 10.1016/j.imbio.2020.151937
Abstrakt: Sand fly saliva presents molecules with potential to development of compounds for treatment of inflammatory diseases. Agaphelin, isolated from the saliva of the mosquito Anopheles gambiae, demonstrates anti-inflammatory properties such as neutrophils chemotaxis inhibition. Here, we extend these results and evaluated the role of agaphelin (0.1-100 nM) in an in vitro model consisting in the activation of human bronchial epithelial cells (BEAS-2B) by IL-4 (50 ng/mL) or lipopolysaccharide (LPS; 10 ng/mL). Agaphelin is non-cytotoxic for BEAS-2B cells. Notably, agaphelin markedly reduces CCL2 and IL-8 production induced by IL-4 or LPS, without altering the IL-10 production. The TLR4 expression and STAT1 phosphorylation induced by LPS were inhibited by agaphlin. In addition, agaphelin decreased the phosphorylation of STAT6 induce by IL-4, whose effect was independent of IL-4-binding activity. Taken together, these findings identify agaphelin as a potential anti-inflammatory therapeutic agent for airway inflammations.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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