Bacteriophage amplification - A comparison of selected methods.

Autor: Skaradzińska A; Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630, Wrocław, Poland. Electronic address: aneta.skaradzinska@upwr.edu.pl., Ochocka M; Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630, Wrocław, Poland., Śliwka P; Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630, Wrocław, Poland., Kuźmińska-Bajor M; Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630, Wrocław, Poland., Skaradziński G; Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630, Wrocław, Poland., Friese A; Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Freie Universitaet Berlin, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany., Roschanski N; Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Freie Universitaet Berlin, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany., Murugaiyan J; Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Freie Universitaet Berlin, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany., Roesler U; Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Freie Universitaet Berlin, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2020 Mar 18; Vol. 282, pp. 113856. Date of Electronic Publication: 2020 Mar 18.
DOI: 10.1016/j.jviromet.2020.113856
Abstrakt: The bactericidal properties of bacteriophages have been used almost since the moment of the discovery of bacterial viruses. In the light of the rapidly growing number of antibiotic-resistant bacteria, phage therapy is considered one of the most promising alternatives to classical treatment. Phage amplification is one of the most common procedures of working with phages, and high-titer preparations are beneficial at the experimental stage of studies as well as in practice. The objective of this study was to compare five commonly applied methods of phage amplification: (i) pooled plaques method, (ii) the plate wash method, (iii) the agar culture method, (iv) the two-stage culture method, and (v) in liquid culture. All methods were tested for fifteen different phages. The results described herein indicate that there is no optimal, universal method for phage amplification, and the most effective method has to be established individually for each phage.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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