| Autor: |
Halabian R; Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran., Moridi K; Department of Medical Nanotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran., Korani M; Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran., Ghollasi M; Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular and cellular medicine [Int J Mol Cell Med] 2019 Winter; Vol. 8 (1), pp. 24-38. Date of Electronic Publication: 2019 Jun 26. |
| DOI: |
10.22088/IJMCM.BUMS.8.1.24 |
| Abstrakt: |
Nanofiber scaffolds and bio-ceramic nanoparticles have been widely used in bone tissue engineering. The use of human- induced pluripotent stem cells (hiPSCs) on this scaffold can be considered as a new approach in the differentiation of bone tissue. In the present study, a polyaniline-gelatin-polycaprolactone (PANi-GEL-PCL) composite nanoscaffold was made by electrospinning and modified superficially by plasma method. The synthesized nanoscaffold was then coated with willemite's bio-ceramic nanoparticles (Zn 2 SiO 4 ). The nanoscaffold's properties were studied by scanning electron microscopy (SEM). Also, nanoparticles characterization was carried out with SEM and dynamic light scattering. The growth and proliferation rate of cells on the synthesized nanoscaffold was examined by MTT assay. Subsequently, hiPSCs were cultured on murine fibroblast cells, incubated in embryoid bodies for 3 days, and placed on the nanoscaffolds. The differentiation potential of hiPSCs was investigated by the examination of common bone markers (e.g. alkaline phosphatase, calcium salt precipitation, and alizarin red test) using bone differentiation factors for 14 days. SEM showed the proper structure of electrospinned nanoscaffolds and coating of nanoparticles on the nanoscaffold surface. The results of MTT assay confirmed the growth and proliferation of cells and the biocompatibility of nanofibers. The results of bone indices also showed that differentiation on the composite nanoscaffold coated with willemite's bio-ceramic nanoparticles dramatically increased in comparison with other groups. Overall, this study demonstrated that PANi-GEL-PCL composite nanoscaffold with willemite's bio-ceramic nanoparticles is a suitable substrate for in vitro growth, proliferation, and differentiation of hiPSCs cells into osteoblasts. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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