Determination of bioavailable phosphorus in water samples using bioassay methods.
| Autor: | Dijkstra ML; Department of Engineering Technology, University of Wisconsin Oshkosh, 800 Algoma Boulevard Oshkosh, WI 54901, USA., Auer MT; Department of Civil and Environmental Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA., Kuczynski A; National Institute of Water and Atmospheric Research, 10 Kyle St., Riccarton, Christchurch 8011, New Zealand., Lambert R; LimnoTech Inc., 501 Avis Dr., Ann Arbor, MI 48108, USA. |
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| Jazyk: | angličtina |
| Zdroj: | MethodsX [MethodsX] 2020 Feb 20; Vol. 7, pp. 100807. Date of Electronic Publication: 2020 Feb 20 (Print Publication: 2020). |
| DOI: | 10.1016/j.mex.2020.100807 |
| Abstrakt: | The total phosphorus analyte (TP) has a long history of use in monitoring and regulatory applications relating to management of cultural eutrophication in freshwaters. It has become apparent, however, that the fraction of the TP analyte ultimately available to support algal growth varies significantly spatially (within a system), seasonally, and among systems. The algal bioassay methods described here provide an approach for determining the bioavailable fraction of the three operationally defined components of TP: soluble reactive phosphorus (SRP), dissolved organic phosphorus (DOP), and particulate phosphorus (PP) in effluents and tributaries discharging to lakes and reservoirs. Application of the technique facilitates a quantitative ranking and targeting of bioavailable phosphorus sources for management.•One congruent method to fractionate particulate and soluble phosphorus (found in aquatic samples) into bioavailable and unavailable fractions was developed based on compilation, adaptation and expansion of two methods from the late 1970s and early 1980s.•Detailed descriptions for culturing phosphorus-starved algae, sub-sampling schedules, kinetics determination, and data presentation are provided•Reproducibility is demonstrated by replication and closure of a mass balance on phosphorus. (© 2020 The Author(s). Published by Elsevier B.V.) |
| Databáze: | MEDLINE |
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