Mass-spectrometry-based draft of the Arabidopsis proteome.

Autor: Mergner J; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany., Frejno M; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany., List M; Chair of Experimental Bioinformatics, Technical University of Munich (TUM), Freising, Germany., Papacek M; Chair of Botany, Technical University of Munich (TUM), Freising, Germany., Chen X; Plant Developmental Biology, Technical University of Munich (TUM), Freising, Germany., Chaudhary A; Plant Developmental Biology, Technical University of Munich (TUM), Freising, Germany., Samaras P; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany., Richter S; Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany., Shikata H; Chair of Plant Systems Biology, Technical University of Munich (TUM), Freising, Germany.; Devision of Plant Environmental Responses, National Institute for Basic Biology, Okazaki, Japan., Messerer M; Plant Genome and Systems Biology, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany., Lang D; Plant Genome and Systems Biology, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany., Altmann S; Institute of Network Biology (INET), Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany., Cyprys P; Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany., Zolg DP; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany., Mathieson T; Cellzome GmbH, Heidelberg, Germany., Bantscheff M; Cellzome GmbH, Heidelberg, Germany., Hazarika RR; Population Epigenetics and Epigenomics, Technical University of Munich (TUM), Freising, Germany.; Institute of Advanced Study (IAS), Technical University of Munich (TUM), Freising, Germany., Schmidt T; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany., Dawid C; Chair of Food Chemistry and Molecular Sensory Science, Technical University of Munich (TUM), Freising, Germany., Dunkel A; Chair of Food Chemistry and Molecular Sensory Science, Technical University of Munich (TUM), Freising, Germany., Hofmann T; Chair of Food Chemistry and Molecular Sensory Science, Technical University of Munich (TUM), Freising, Germany., Sprunck S; Cell Biology and Plant Biochemistry, University of Regensburg, Regensburg, Germany., Falter-Braun P; Institute of Network Biology (INET), Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany.; Chair of Microbe-Host Interactions, Ludwigs-Maximilians-University (LMU), Munich, Germany., Johannes F; Population Epigenetics and Epigenomics, Technical University of Munich (TUM), Freising, Germany.; Institute of Advanced Study (IAS), Technical University of Munich (TUM), Freising, Germany., Mayer KFX; Plant Genome and Systems Biology, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany.; Plant Genome Biology, Technical University of Munich (TUM), Freising, Germany., Jürgens G; Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany., Wilhelm M; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany., Baumbach J; Chair of Experimental Bioinformatics, Technical University of Munich (TUM), Freising, Germany., Grill E; Chair of Botany, Technical University of Munich (TUM), Freising, Germany., Schneitz K; Plant Developmental Biology, Technical University of Munich (TUM), Freising, Germany., Schwechheimer C; Chair of Plant Systems Biology, Technical University of Munich (TUM), Freising, Germany., Kuster B; Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM), Freising, Germany. kuster@tum.de.; Institute of Advanced Study (IAS), Technical University of Munich (TUM), Freising, Germany. kuster@tum.de.; Bavarian Biomolecular Mass Spectrometry Center (BayBioMS), Technical University of Munich (TUM), Freising, Germany. kuster@tum.de.
Jazyk: angličtina
Zdroj: Nature [Nature] 2020 Mar; Vol. 579 (7799), pp. 409-414. Date of Electronic Publication: 2020 Mar 11.
DOI: 10.1038/s41586-020-2094-2
Abstrakt: Plants are essential for life and are extremely diverse organisms with unique molecular capabilities 1 . Here we present a quantitative atlas of the transcriptomes, proteomes and phosphoproteomes of 30 tissues of the model plant Arabidopsis thaliana. Our analysis provides initial answers to how many genes exist as proteins (more than 18,000), where they are expressed, in which approximate quantities (a dynamic range of more than six orders of magnitude) and to what extent they are phosphorylated (over 43,000 sites). We present examples of how the data may be used, such as to discover proteins that are translated from short open-reading frames, to uncover sequence motifs that are involved in the regulation of protein production, and to identify tissue-specific protein complexes or phosphorylation-mediated signalling events. Interactive access to this resource for the plant community is provided by the ProteomicsDB and ATHENA databases, which include powerful bioinformatics tools to explore and characterize Arabidopsis proteins, their modifications and interactions.
Databáze: MEDLINE
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