Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli -Induced Injury.

Autor: Silva TF; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Cavalcanti Filho JRN; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil.; Faculdade de Odontologia, Universidade de Pernambuco, Camaragibe/PE 54756-220, Brazil., Barreto Fonsêca MML; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil.; Curso de Farmácia, Faculdade Pernambucana de Saúde, Recife/PE 51150-000, Brazil., Santos NMD; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Barbosa da Silva AC; Curso de Biomedicina, Universidade Ceuma, São Luís/MA 65075-120, Brazil., Zagmignan A; Curso de Nutrição, Universidade Ceuma, São Luís/MA 65075-120, Brazil.; Programas de Pós-graduação, Universidade Ceuma, São Luís/MA 65075-120, Brazil., Abreu AG; Programas de Pós-graduação, Universidade Ceuma, São Luís/MA 65075-120, Brazil., Sant'Anna da Silva AP; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Lima VLM; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Silva NHD; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Dutra LM; Núcleo de Estudos e Pesquisas de Plantas Medicinais, Universidade Federal do Vale do São Francisco, Petrolina/PE 56304-205, Brazil., Almeida JRGDS; Núcleo de Estudos e Pesquisas de Plantas Medicinais, Universidade Federal do Vale do São Francisco, Petrolina/PE 56304-205, Brazil., Silva MVD; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Correia MTDS; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife/PE 50670-901, Brazil., Nascimento da Silva LC; Curso de Biomedicina, Universidade Ceuma, São Luís/MA 65075-120, Brazil.; Programas de Pós-graduação, Universidade Ceuma, São Luís/MA 65075-120, Brazil.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2020 Mar 16; Vol. 13 (3). Date of Electronic Publication: 2020 Mar 16.
DOI: 10.3390/ph13030046
Abstrakt: The relevance of oxidative stress in the pathogenesis of several diseases (including inflammatory disorders) has traditionally led to the search for new sources of antioxidant compounds. In this work, we report the selection of fractions with high antioxidant action from B. tetraphylla (BT) leaf extracts. In vitro methods (DPPH and ABTS assays; determination of phenolic and flavonoid contents) were used to select products derived from B. tetraphylla with high antioxidant action. Then, the samples with the highest potentials were evaluated in a model of injury based on the inoculation of a lethal dose of heat-inactivated Escherichia coli in Tenebrio molitor larvae. Due to its higher antioxidant properties, the methanolic extract (BTME) was chosen to be fractionated using Sephadex LH-20 column-based chromatography. Two fractions from BTME (BTFC and BTFD) were the most active fractions. Pre-treatment with these fractions protected larvae of T. molitor from the stress induced by inoculation of heat-inactivated E. coli . Similarly, BTFC and BTFD increased the lifespan of larvae infected with a lethal dose of enteroaggregative E. coli 042. NMR data indicated the presence of aliphatic compounds (terpenes, fatty acids, carbohydrates) and aromatic compounds (phenolic compounds). These findings suggested that products derived from B. tetraphylla leaves are promising candidates for the development of antioxidant and anti-infective agents able to treat oxidative-related dysfunctions.
Databáze: MEDLINE
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