Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

Autor: Kachkoul R; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco.; Faculty of Science and Technology, Sidi Mohammed Ben Abdellah University, BP 2202, Road of Imouzzer, Fez, Morocco., Houssaini TS; Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco.; Department of Nephrology, University of Hospital Hassan II, BP 1835, Atlas, Road of Sidi Harazem, Fez, Morocco., Mohim M; Faculty of Science and Technology, Sidi Mohammed Ben Abdellah University, BP 2202, Road of Imouzzer, Fez, Morocco.; Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco., El Habbani R; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco., Lahrichi A; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2020 Feb 24; Vol. 2020, pp. 9424510. Date of Electronic Publication: 2020 Feb 24 (Print Publication: 2020).
DOI: 10.1155/2020/9424510
Abstrakt: The plant Punica granatum L. has several biological activities and a great curative and preventive power against chronic diseases. For this purpose, the objective of this work is to valorize the fruit peel of this plant in the field of phytomedicine, by quantifying and identifying its bioactive compounds and by evaluating their antioxidant and anticrystallization activities against calcium oxalate. This comparative study has been carried out by hydroalcoholic extract (E.PG) and infusion (I.PG) of the plant. The quantification of the phenolic compounds has been performed by spectrophotometric methods, and the chemical species identification has been performed by UPLC-PDA-ESI-MS. Moreover, the examination of the antioxidant activity has been executed by both methods of DPPH and FRAP. The crystallization inhibition has been studied in vitro by the turbidimetric model. The characterization of the synthesized crystals has been accomplished by microscopic observation and by Fourier Transform Infrared Spectroscopy. The results found show the comparable importance of the two plant extracts in the elimination of free radicals; the values of the half maximal inhibitory concentration "IC 50 " obtained are in the order of 60.87 ± 0.27 and 59.91 ± 0.83  μ g/mL by the DPPH method and in the order of 42.17 ± 7.46 and 79.77 ± 6.91  μ g/mL by the FRAP method, for both E.PG and I.PG, respectively. Furthermore, the inhibition percentages of calcium oxalate crystallization are in the range of 98.11 ± 0.17 and 98.22 ± 0.71% against the nucleation and in the order of 88.98 ± 0.98 and 88.78 ± 2.48% against the aggregation, for E.PG and I.PG, respectively. These results prove the richness of the plant in bioactive compounds, offering an antioxidant and anticrystallization capacity; therefore, it can be used in the treatment and/or the prevention of stone formation.
Competing Interests: The authors wish to confirm that there are no known conflicts of interest associated with this publication.
(Copyright © 2020 Rabie Kachkoul et al.)
Databáze: MEDLINE
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