Local Mast Cell Activation Promotes Neovascularization.

Autor: Bot I; Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University,2333 CC, Leiden, The Netherlands., Velden DV; Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University,2333 CC, Leiden, The Netherlands., Bouwman M; Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University,2333 CC, Leiden, The Netherlands.; Department of Surgery, Leiden University Medical Center, 2300RC Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Kröner MJ; Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University,2333 CC, Leiden, The Netherlands., Kuiper J; Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University,2333 CC, Leiden, The Netherlands., Quax PHA; Department of Surgery, Leiden University Medical Center, 2300RC Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2300RC Leiden, The Netherlands., de Vries MR; Department of Surgery, Leiden University Medical Center, 2300RC Leiden, The Netherlands.; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2300RC Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: Cells [Cells] 2020 Mar 12; Vol. 9 (3). Date of Electronic Publication: 2020 Mar 12.
DOI: 10.3390/cells9030701
Abstrakt: Mast cells have been associated with arteriogenesis and collateral formation. In advanced human atherosclerotic plaques, mast cells have been shown to colocalize with plaque neovessels, and mast cells have also been associated with tumor vascularization. Based on these associations, we hypothesize that mast cells promote angiogenesis during ischemia. In human ischemic muscle tissue from patients with end-stage peripheral artery disease, we observed activated mast cells, predominantly located around capillaries. Also, in mouse ischemic muscles, mast cells were detected during the revascularization process and interestingly, mast cell activation status was enhanced up to 10 days after ischemia induction. To determine whether mast cells contribute to both arteriogenesis and angiogenesis, mast cells were locally activated immediately upon hind limb ischemia in C57Bl/6 mice. At day 9, we observed a 3-fold increase in activated mast cell numbers in the inguinal lymph nodes. This was accompanied by an increase in the amount of Ly6C high inflammatory monocytes. Interestingly, local mast cell activation increased blood flow through the hind limb (46% at day 9) compared to that in non-activated control mice. Histological analysis of the muscle tissue revealed that mast cell activation did not affect the number of collaterals, but increased the collateral diameter, as well as the number of CD31 + capillaries. Together, these data illustrate that locally activated mast cell contribute to arteriogenesis and angiogenesis.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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