Regulatory effect of PGE 2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis.

Autor: Venturin GL; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Bragato JP; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Melo LM; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Rebech GT; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Costa SF; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., de Siqueira CE; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Oliveira Dos Santos Maciel M; School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Eugênio FR; Clinics Department, Animal Surgery and Reproduction, School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., Patto Santos PS; Clinics Department, Animal Surgery and Reproduction, School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil., de Lima VMF; Clinics Department, Animal Surgery and Reproduction, School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA), Sao Paulo State University 'Júlio de Mesquita Filho'(UNESP), Araçatuba, Brazil.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2020 Jun; Vol. 42 (6), pp. e12713. Date of Electronic Publication: 2020 Apr 06.
DOI: 10.1111/pim.12713
Abstrakt: Canine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE 2 ) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE 2 and PGE 2 receptor levels and the regulatory effect of PGE 2 on arginase activity, NO 2 , IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO 2 levels decreased when the cells were treated with a PGE 2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE 2 receptor agonist (EP2) or PGE 2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE 2 . We conclude that Leishmania infection of dogs modulates PGE 2 receptors and speculate that the binding of PGE 2 to its receptors may activate the microbicidal capacity of cells.
(© 2020 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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