Aerobic Exercise Training Induces the Mitonuclear Imbalance and UPRmt in the Skeletal Muscle of Aged Mice.

Autor:	Cordeiro AV; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Brícola RS; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Braga RR; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Lenhare L; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Silva VRR; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Anaruma CP; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil.; Department of Physical Education, Institute of Biosciences, São Paulo State University, Rio Claro, Brazil., Katashima CK; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Crisol BM; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil., Simabuco FM; Laboratory of Functional Properties in Foods, University of Campinas, Limeira, Brazil., Silva ASR; Postgraduate Program in Rehabilitation and Functional Performance, University of São Paulo, Ribeirão Preto, Brazil.; School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Cintra DE; Laboratory of Nutritional Genomics, University of Campinas, Limeira, Brazil., Moura LP; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil.; Department of Physical Education, Institute of Biosciences, São Paulo State University, Rio Claro, Brazil.; CEPECE - Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Brazil., Pauli JR; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil.; CEPECE - Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Brazil., Ropelle ER; Laboratory of Molecular Biology of Exercise, University of Campinas, Limeira, Brazil.; CEPECE - Center of Research in Sport Sciences, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, Brazil.; Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil.
Jazyk:	angličtina
Zdroj:	The journals of gerontology. Series A, Biological sciences and medical sciences [J Gerontol A Biol Sci Med Sci] 2020 Nov 13; Vol. 75 (12), pp. 2258-2261.
DOI:	10.1093/gerona/glaa059
Abstrakt:	The impairment of the mitochondrial functions is a hallmark of aging. During aging, there is a downregulation of two mechanisms strictly associated with mitochondrial integrity, including the mitonuclear imbalance (eg, imbalance in mitochondrial- versus nuclear-encoded mitochondrial proteins) and the mitochondrial unfolded protein response (UPRmt). Here, we evaluated the effects of aerobic exercise in the mitonuclear imbalance and UPRmt markers in the skeletal muscle of old mice. We combined the physiological tests, molecular and bioinformatic analyzes to evaluate the effects of 4 weeks of aerobic exercise training on mitonuclear imbalance and UPRmt markers in the skeletal muscle of young (2 months) and aged (24 months) C57BL/6J mice. Initially, we found that aging reduced several mitochondrial genes in the gastrocnemius muscle, and it was accompanied by the low levels of UPRmt markers, including Yme1l1 and Clpp mRNA. As expected, physical training improved the whole-body metabolism and physical performance of aged mice. The aerobic exercise increased key proteins involved in the mitochondrial biogenesis/functions (VDAC and SIRT1) along with mitochondrial-encoded genes (mtNd1, mtCytB, and mtD-Loop) in the skeletal muscle of old mice. Interestingly, aerobic exercise induced the mitonuclear imbalance, increasing MTCO1/ATP5a ratio and UPRmt markers in the skeletal muscle, including HSP60, Lonp1, and Yme1L1 protein levels in the gastrocnemius muscle of aged mice. These data demonstrate that aerobic exercise training induced mitonuclear imbalance and UPRmt in the skeletal muscle during aging. These phenomena could be involved in the improvement of the mitochondrial metabolism and oxidative capacity in aged individuals. (© The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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