Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.

Autor: Frayssinet A; Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France., Petta D; AO Research Institute Davos, Davos Platz, Switzerland., Illoul C; Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France., Haye B; Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France., Markitantova A; Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France., Eglin D; AO Research Institute Davos, Davos Platz, Switzerland., Mosser G; Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France., D'Este M; AO Research Institute Davos, Davos Platz, Switzerland., Hélary C; Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France. Electronic address: christophe.helary@upmc.fr.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2020 May 15; Vol. 236, pp. 116042. Date of Electronic Publication: 2020 Feb 20.
DOI: 10.1016/j.carbpol.2020.116042
Abstrakt: A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties. At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels. At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed. These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration. Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability. These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils. Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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